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Literature summary for 3.2.2.21 extracted from
- Effects of substrate specificity on initiating the base excision repair of N-methylpurines by variant human 3-methyladenine DNA glycosylases (2005), Chem. Res. Toxicol., 18, 87-94.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| expression in Saccharomyces cerevisiae | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N169A | ratio of 3-methyladenine/7-methylguanine is 29:1 for wild-type enzyme and 26:1 for mutant enzyme N169A, 30fold lower activity than wild-type enzyme. Expression of the N169A in Saccharomyces cerevisiae during methyl methanesulfonate exposure results in greater sensitivity, greater mutation induction following exposure to methyl methanesulfonate and more strand breaks in vivo | Homo sapiens |
| N169D | ratio of 3-methyladenine/7-methylguanine is 29:1 for wild-type enzyme, 100fold lower production of 3-methyladeine and no production of 7-methylguanine is detected with mutant enzyme N169D. When expressed in Saccharomyces cerevisiae, the N169D variant provides better protection against methyl methanesulfonate toxicity than wild-type enzyme. Fewer strand breaks in vivo are seen in the presence of the N169D variant following exposure to methyl methanesulfonate | Homo sapiens |
| N169S | ratio of 3-methyladenine/7-methylguanine is 29:1 for wild-type enzyme and 24:1 for mutant enzyme N169S, 30fold lower activity than wild-type enzyme. Expression of the N169S in Saccharomyces cerevisiae during methyl methanesulfonate exposure results in greater sensitivity, greater mutation induction following exposure to methyl methanesulfonate and more strand breaks in vivo | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alkylated DNA + H2O | - |
Homo sapiens | 3-methyladenine + 7-methylguanine + ? | ratio of 3-methyladenine/7-methylguanine is 29:1 for wild-type enzyme, 24:1 for mutant enzyme N169S and 26:1 for mutant enzyme N169A, no production of 7-methylguanine is detected with mutant enzyme N169D | ? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AAG | - |
Homo sapiens |
| ANPG | - |
Homo sapiens |
| MPG | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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