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Literature summary for 3.2.2.21 extracted from

  • Karran, P.; Hjelmgren, T.; Lindahl, T.
    Induction of a DNA glycosylase for N-methylated purines is part of the adaptive response to alkylating agents (1982), Nature, 296, 770-773.
    View publication on PubMed
Search for more literature for 3.2.2.21

Activating Compound

Activating Compound Comment Organism Structure
N-methyl-N'-nitro-N-nitrosoguanidine MNNG, good inducer of the enzyme, methylmethanesulfonate is less effective and 4-nitroquinoline-1-oxide has no effect Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
additional information resistant to product inhibition by free 3-methyladenine Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alkylated DNA + H2O Escherichia coli
-
 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 tagA mutant BK2114
-
no activity in Escherichia coli
-
 alk mutants
-

Purification (Commentary)

Purification (Comment) Organism
partially Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alkylated DNA + H2O
-
 Escherichia coli 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
 ?

Synonyms

Synonyms Comment Organism
m3A DNA glycosylase II
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48
-
 relatively heat resistant Escherichia coli