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Literature summary for 3.2.2.2 extracted from

  • Elshafei, A.; Mohamed, L.; Hassan, M.
    Degradation of purine ribonucleosides by extracts of Penicillium viridicatum (2011), Afr. J. Microbiol. Res., 5, 316-323.
No PubMed abstract available
Search for more literature for 3.2.2.2

General Stability

General Stability Organism
hydrolysis activity for inosine, guanosine and adenosine is highest in citrate-phosphate buffer Penicillium viridicatum

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 24.2% residual activity at 0.1 M using inosine as substrate, 25.8% residual activity at 0.1 M using guanosine as substrate, 33.3% residual activity at 0.1 M using adenosine as substrate Penicillium viridicatum
Cu2+ 10.6% residual activity at 0.1 M using inosine as substrate, 9.67% residual activity at 0.1 M using guanosine as substrate, complete inhibition at 0.1 M using adenosine as substrate Penicillium viridicatum
Fe2+ 60.6% residual activity at 0.1 M using inosine as substrate, 54.8% residual activity at 0.1 M using guanosine as substrate, 66.6% residual activity at 0.1 M using adenosine as substrate Penicillium viridicatum
Mg2+ 62.1% residual activity at 0.1 M using inosine as substrate, 74.2% residual activity at 0.1 M using guanosine as substrate, 75% residual activity at 0.1 M using adenosine as substrate Penicillium viridicatum
Mn2+ complete inhibition at 0.1 M using inosine, adenosine or guanosine as substrate Penicillium viridicatum
NaCN 60.6% residual activity at 0.1 M using inosine as substrate, 38.7% residual activity at 0.1 M using guanosine as substrate, 41.6% residual activity at 0.1 M using adenosine as substrate Penicillium viridicatum
Zn2+ 57.5% residual activity at 0.1 M using inosine as substrate, 45.2% residual activity at 0.1 M using guanosine as substrate, 41.6% residual activity at 0.1 M using adenosine as substrate Penicillium viridicatum

Organism

Organism UniProt Comment Textmining
Penicillium viridicatum
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-
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Penicillium viridicatum NRC 3712
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-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:nitrate-grown cell
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 Penicillium viridicatum
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Storage Stability

Storage Stability Organism
-5°C, in citrate-phosphate buffer (pH 4.0), the enzyme retains 100%, 95.7%, 93,6% and 82.9% activity for inosine hydrolysis after 24 h, 48 h, 72 h and 120 h of incubation, respectively Penicillium viridicatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine + H2O after 1 h, the rate of adenosine cleavage is about 12.5% Penicillium viridicatum D-ribose + adenine
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 ?
adenosine + H2O after 1 h, the rate of adenosine cleavage is about 12.5% Penicillium viridicatum NRC 3712 D-ribose + adenine
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 ?
guanosine + H2O after 1 h, the rate of guanosine cleavage is about 16% Penicillium viridicatum D-ribose + guanine
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 ?
guanosine + H2O after 1 h, the rate of guanosine cleavage is about 16% Penicillium viridicatum NRC 3712 D-ribose + guanine
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 ?
inosine + H2O best substrate Penicillium viridicatum D-ribose + hypoxanthine
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 ?
inosine + H2O best substrate Penicillium viridicatum NRC 3712 D-ribose + hypoxanthine
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 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Penicillium viridicatum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 the enzyme shows 68.18%, 45.45% and 28.78% residual activities for inosine hydrolysis after 4 min, 6 min, and 8 min of incubation, respectively, at 50°C. The enzyme is completely inactivated after 10 min at 50°C and 4 min at 60°C Penicillium viridicatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Penicillium viridicatum