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Literature summary for 3.2.2.2 extracted from
- Inosine nucleosidase from Azotobacter vinelandii. Purification and properties (1978), Arch. Microbiol., 119, 59-64.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenine | partial noncompetitive | Azotobacter vinelandii |  |
| hypoxanthine | partial noncompetitive | Azotobacter vinelandii | |
| nucleoside diphosphates | less effective inhibitor compared to nucleoside triphosphates, non competitive inhibition | Azotobacter vinelandii | |
| nucleoside monophosphates | slight inhibition or no effect | Azotobacter vinelandii | |
| nucleoside triphosphates | most potent inhibitor, non competitive inhibition | Azotobacter vinelandii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.65 | - |
Inosine | pH 7.1 | Azotobacter vinelandii | |
| 0.85 | - |
Inosine | pH 9.0 | Azotobacter vinelandii | |
| 1.2 | - |
Xanthosine | pH 7.1 | Azotobacter vinelandii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
gel filtration | Azotobacter vinelandii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inosine + H2O | Azotobacter vinelandii | - |
hypoxanthine + D-ribose | - |
? | |
| inosine + H2O | Azotobacter vinelandii 0 | - |
hypoxanthine + D-ribose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Azotobacter vinelandii | - |
- |
- |
| Azotobacter vinelandii 0 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| highly purified, 233fold | Azotobacter vinelandii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.33 | - |
- |
Azotobacter vinelandii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenosine + H2O | - |
Azotobacter vinelandii | adenine + D-ribose | - |
? | |
| adenosine + H2O | - |
Azotobacter vinelandii 0 | adenine + D-ribose | - |
? | |
| guanosine + H2O | - |
Azotobacter vinelandii | guanine + D-ribose | - |
? | |
| guanosine + H2O | - |
Azotobacter vinelandii 0 | guanine + D-ribose | - |
? | |
| inosine + H2O | - |
Azotobacter vinelandii | hypoxanthine + D-ribose | - |
? | |
| inosine + H2O | - |
Azotobacter vinelandii 0 | hypoxanthine + D-ribose | - |
? | |
| xanthosine + H2O | - |
Azotobacter vinelandii | xanthine + D-ribose | - |
? | |
| xanthosine + H2O | - |
Azotobacter vinelandii 0 | xanthine + D-ribose | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
substrate: xanthosine | Azotobacter vinelandii |
| 9 | - |
substrate: inosine | Azotobacter vinelandii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | - |
Azotobacter vinelandii |
| 9 | - |
no xanthosine hydrolysis above this temperature | Azotobacter vinelandii |
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