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Literature summary for 3.2.2.19 extracted from
- Amino acid specific ADP-ribosylation: substrate specificity of an ADP-ribosylarginine hydrolase from turkey erythrocytes (1986), Biochemistry, 25, 5408-5414.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | inhibition by ADP stronger than by AMP | Meleagris gallopavo |  |
| ADPribose | inhibition by ADPribose stronger than by ADP and AMP | Meleagris gallopavo | |
| alphaNAD+ | inhibition by ADPribose stronger than by alphaNAD+ and betaNAD+ | Meleagris gallopavo | |
| AMP | - |
Meleagris gallopavo | |
| betaNAD+ | inhibition by alphaNAD+ stronger than by betaNAD+ | Meleagris gallopavo | |
| dithiothreitol | incubation in 20 mM at 37°C leads to rapid loss of activity but addition of Mg2+ stabilizes the hydrolase against thermal inactivation | Meleagris gallopavo | |
| NaCl | inhibition with 200 mM | Meleagris gallopavo | |
| NaF | more than 80% inhibition with 5 mM | Meleagris gallopavo | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0271 | - |
ADP-ribosylguanidine | - |
Meleagris gallopavo | |
| 0.0472 | - |
2'-phospho-ADP-ribosylarginine | - |
Meleagris gallopavo | |
| 0.065 | - |
ADP-ribosylarginine | - |
Meleagris gallopavo | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
- |
Meleagris gallopavo |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-ribose-arginine + H2O | Meleagris gallopavo | the presence of different enzymes and effectors controlling ADP-ribosylarginine synthesis and degradation is reminiscent of the protein kinase-phosphatase system | ADP-ribose + arginine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Meleagris gallopavo | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 800fold by DE-52, phenyl-Sepharose, carboxymethylcellulose, organomercurial agarose, Ultrogel AcA 54 | Meleagris gallopavo |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Meleagris gallopavo | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0000476 | - |
- |
Meleagris gallopavo |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-phospho-ADP-ribosylarginine + H2O | - |
Meleagris gallopavo | 2'-phospho-ADP-ribose + arginine | - |
? | |
| ADP-ribose-arginine + H2O | the presence of different enzymes and effectors controlling ADP-ribosylarginine synthesis and degradation is reminiscent of the protein kinase-phosphatase system | Meleagris gallopavo | ADP-ribose + arginine | - |
? | |
| ADP-ribose-L-arginine + H2O | - |
Meleagris gallopavo | ADP-ribose + L-arginine | - |
? | |
| ADP-ribosylarginine + H2O | - |
Meleagris gallopavo | ADP-ribose + L-arginine | - |
? | |
| ADP-ribosylguanidine + H2O | at pH less than 7, the enzme exhibits more activity toward ADP-ribosylguanidine than toward ADP-ribosylarginine. The ratio of activity in assays containing ADP-ribosylarginine to those with ADP-ribosylguanidine increases with pH | Meleagris gallopavo | ADP-ribose + guanidine | - |
? | |
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