Any feedback?
Literature summary for 3.2.2.17 extracted from
- Enzymatic repair of pyrimidine dimer-containing DNA. A 5' dimer DNA glycosylase: 3'-apyrimidinic endonuclease mechanism from Micrococcus luteus (1982), J. Biol. Chem., 257, 13465-13474.
General Stability
| General Stability | Organism |
|---|---|
| resistant to EDTA | Micrococcus luteus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000015 | - |
DNA | thymine labeled DNA | Micrococcus luteus |  |
| 0.00028 | - |
DNA | cytosine labeled DNA | Micrococcus luteus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17500 | - |
SDS-PAGE | Micrococcus luteus |
| 18000 | - |
- |
Micrococcus luteus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Micrococcus luteus | only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Micrococcus luteus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Micrococcus luteus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| stable on ice for 8 months with no loss in activity | Micrococcus luteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA phiX174 RFI + H2O | irradiated with increasing doses of UV light | Micrococcus luteus | thymine + ? | - |
? | |
| additional information | only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded | Micrococcus luteus | ? | - |
? | |
| oligonucleotides + H2O | - |
Micrococcus luteus | dCMP + dTMP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 17500-18000, single polypeptide chain | Micrococcus luteus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5'dimer DNA glycosylase | - |
Micrococcus luteus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 55 | thermal inactivation of both endonuclease and glycosylase activity | Micrococcus luteus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
