Activating Compound | Comment | Organism | Structure |
---|---|---|---|
isopropyl-1-thio-beta-D-galactopyranoside | induction of the pdg gene | Paramecium bursaria Chlorella virus 1 |
Cloned (Comment) | Organism |
---|---|
gene cv-pdg encoding T4 endonucleaseV homolog, 41% identical to T4endoV, enhance UV radiation resistance in repair-deficient Escherichia coli BL21, vector pCRII, cloning and overexpression of the gene product, cloned into the Escherichia coli expression plasmid pET11a | Paramecium bursaria Chlorella virus 1 |
Crystallization (Comment) | Organism |
---|---|
- |
Tequatrovirus T4 |
General Stability | Organism |
---|---|
relatively stable and soluble under most conditions | Paramecium bursaria Chlorella virus 1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | above 40 mM | Tequatrovirus T4 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000014 | - |
DNA | cis-syn 49-bp duplex | Paramecium bursaria Chlorella virus 1 | |
0.00000065 | - |
DNA | trans-syn 49-bp duplex | Paramecium bursaria Chlorella virus 1 | |
0.00000267 | - |
DNA | cis-syn 12-bp duplex | Paramecium bursaria Chlorella virus 1 | |
0.000005 | - |
DNA | wild-type | Tequatrovirus T4 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | slightly more stimulatory than NaCl | Paramecium bursaria Chlorella virus 1 | |
NaCl | - |
Micrococcus luteus | |
NaCl | - |
Tequatrovirus T4 | |
NaCl | optimal activity at 100-120 mM | Paramecium bursaria Chlorella virus 1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
- |
Tequatrovirus T4 |
16000 | - |
- |
Paramecium bursaria Chlorella virus 1 |
18000 | - |
- |
Tequatrovirus T4 |
18000 | - |
uveA, 27% amino acid identity with T4 endonuclease V | Micrococcus luteus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Micrococcus luteus | only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded | ? | - |
? | |
additional information | Tequatrovirus T4 | only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded | ? | - |
? | |
additional information | Paramecium bursaria Chlorella virus 1 | only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrococcus luteus | - |
- |
- |
Paramecium bursaria Chlorella virus 1 | - |
eukaryotic algal virus, Chlorella virus, PBCV-1 | - |
Tequatrovirus T4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Paramecium bursaria Chlorella virus 1 |
purified from Escherichia coli AB2480 | Tequatrovirus T4 |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
infected cell | infected Chlorella NC64A | Paramecium bursaria Chlorella virus 1 | - |
Storage Stability | Organism |
---|---|
-80°C stable when stored in 50% glycerol, -20°C stable for several freeze-thaw cycles, 4°C in buffer without glycerol for up to a year without loss of activity | Paramecium bursaria Chlorella virus 1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Micrococcus luteus | thymine + ? | - |
? | |
DNA + H2O | - |
Tequatrovirus T4 | thymine + ? | - |
? | |
DNA + H2O | cis-syn pyrimidine dimer specific | Paramecium bursaria Chlorella virus 1 | thymine + ? | - |
? | |
additional information | only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded | Micrococcus luteus | ? | - |
? | |
additional information | only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded | Tequatrovirus T4 | ? | - |
? | |
additional information | only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded | Paramecium bursaria Chlorella virus 1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Chlorella virus pyrimidine dimer glycosylase | cv-PDG | Paramecium bursaria Chlorella virus 1 |
pyrimidine dimer-specific glycosylase/AP lyase | - |
Paramecium bursaria Chlorella virus 1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
thermostable, difficult to completely heat inactivate, retains 10% activity after 20 min at 90°C | Paramecium bursaria Chlorella virus 1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | pH optimum for the 2 enzymatic activities differs, AP lyase function at pH 6.5, becoming inactive at pH 8 | Tequatrovirus T4 |
6 | 8 | pH optimum for the 2 enzymatic activities differs, AP lyase function at pH 6.5, becoming inactive at pH 8 | Paramecium bursaria Chlorella virus 1 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Paramecium bursaria Chlorella virus 1 |