Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DNA | depurinated DNA competitively inhibits the UV endonuclease activity, irradiated DNA inhibits the AP endonuclease activity | Micrococcus luteus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | - |
Tequatrovirus T4 | |
NaCl | stimulated by the presence of 50-100 mM salt | Micrococcus luteus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
Stokes radius | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Micrococcus luteus | only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded | ? | - |
? | |
additional information | Tequatrovirus T4 | only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrococcus luteus | - |
- |
- |
Tequatrovirus T4 | - |
T4amN82, host Escherichia coli B/5 | - |
Purification (Comment) | Organism |
---|---|
- |
Micrococcus luteus |
- |
Tequatrovirus T4 |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
infected cell | cells infected with wild-type phage T4 | Tequatrovirus T4 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded | Micrococcus luteus | ? | - |
? | |
additional information | only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded | Tequatrovirus T4 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
denV | - |
Tequatrovirus T4 |
endonuclease V | - |
Tequatrovirus T4 |
T4 PD-DNA-glycosylase | - |
Tequatrovirus T4 |
T4-induced UV endonuclease | - |
Tequatrovirus T4 |
UV-specific endonuclease | - |
Tequatrovirus T4 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
46 | - |
2 enzyme activities resides in the same protein molecule, activities lost in parallel during incubation for up to 17 min at 46°C | Tequatrovirus T4 |
50 | - |
UV endonuclease and AP endonuclease inactivated in parallel at 50°C | Micrococcus luteus |