Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Tequatrovirus T4 | UV-irradiated DNA, containing thymine dimers | ? | - |
? | |
additional information | Tequatrovirus T4 | only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
bacteriophage, Escherichia coli cells infected with wild-type v+ | - |
Purification (Comment) | Organism |
---|---|
partially | Tequatrovirus T4 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
thymidine-dimer-containing DNA + H2O = thymidine-dimer-containing DNA with apyrimidinic site | hydrolysis of phosphodiester bonds | Tequatrovirus T4 |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
infected cell | infected E. coli with phage T4v+ | Tequatrovirus T4 | - |
infected cell | cells infected with wild-type phage T4 | Tequatrovirus T4 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | UV-irradiated DNA, containing thymine dimers | Tequatrovirus T4 | thymine + ? | enzymatic photoreactivation, direct photoreversal, releases free thymine from E. coli DNA | ? | |
DNA + H2O | UV-irradiated DNA, containing thymine dimers | Tequatrovirus T4 | ? | - |
? | |
additional information | only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded | Tequatrovirus T4 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
denV | - |
Tequatrovirus T4 |
PD-specific DNA glycosylase | - |
Tequatrovirus T4 |
T4 endonuclease V | - |
Tequatrovirus T4 |
T4 PD-DNA-glycosylase | - |
Tequatrovirus T4 |
T4 UV endonuclease | - |
Tequatrovirus T4 |