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Literature summary for 3.2.2.17 extracted from

  • Nakabeppu, Y.; Yamashita, K.; Sekiguchi, M.
    Purification and characterization of normal and mutant forms of T4 endonuclease V (1982), J. Biol. Chem., 257, 2556-2562.
    View publication on PubMed
Search for more literature for 3.2.2.17

Inhibitors

Inhibitors Comment Organism Structure
NaCl nicking activity rather inhibited Tequatrovirus T4

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl activity to produce alkali-labile sites stimulated by 25-100 mM Tequatrovirus T4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
-
 Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Tequatrovirus T4 only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded ?
-
 ?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
 T4 D, T4v1, T4uvs-5, T4uvs-13, host Escherichia coli B, Escherichia coli 1100, Escherichia coli Cr63
-

Purification (Commentary)

Purification (Comment) Organism
-
 Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded Tequatrovirus T4 ?
-
 ?
poly(dAdT) + H2O
-
 Tequatrovirus T4 oligonucleotides acid-soluble oligonucleotides, length less than 15 nucleotides ?

Subunits

Subunits Comment Organism
monomer 1 * 17500-18000, single polypeptide chain Tequatrovirus T4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Tequatrovirus T4

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5
-
 Tequatrovirus T4