Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21trxB cells | Mycobacterium tuberculosis |
gene RV0091, recombinant expression of active His-tagged enzyme in Escherichia coli strain BL21trxB, optimization of expression method | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-methyl-5'-thioadenosine + H2O | Mycobacterium tuberculosis | - |
S-methyl-5-thio-D-ribose + adenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis | P9WJN3 | gene RV0091 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, and gel filtration | Mycobacterium tuberculosis |
recombinant His-tagged enzyme from Escherichia coli strain BL21trxB by nickel affinity chromatography to over 95% purity | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + H2O | - |
Mycobacterium tuberculosis | adenine + S-methyl-5-thio-D-ribose | - |
? | |
S-methyl-5'-thioadenosine + H2O | - |
Mycobacterium tuberculosis | S-methyl-5-thio-D-ribose + adenine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Mycobacterium tuberculosis |
5-methylthioadenosine nucleosidase | - |
Mycobacterium tuberculosis |
MTAN | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Mycobacterium tuberculosis |
37 | - |
recombinant His-tagged enzyme | Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the purified recombinant His-tagged enzyme shows a poor thermal stability | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | 12 | - |
Mycobacterium tuberculosis |
10 | 12 | recombinant His-tagged enzyme | Mycobacterium tuberculosis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 12 | recombinant His-tagged enzyme, stable at, denaturation and loss of activity at and below pH 7.0 | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme plays a key role in the metabolism of Mycobacterium tuberculosis | Mycobacterium tuberculosis |