Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
expressed in Escherichia coli BL21(DE3) cells | Salmonella enterica |
expressed in Escherichia coli BL21(DE3) cells | Vibrio cholerae serotype O1 |
recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21 Star(DE3) | Escherichia coli |
recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21 Star(DE3) | Vibrio cholerae serotype O1 |
Crystallization (Comment) | Organism |
---|---|
crystal structure analysis, PDB entry 1Y6Q and PDB entry 1Z5P | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(4S,5S)-2-(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)-5-[(methylsulfanyl)methyl]-1,2-thiazolidin-4-ol | - |
Salmonella enterica | |
(4S,5S)-2-(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)-5-[(methylsulfanyl)methyl]-1,2-thiazolidin-4-ol | - |
Vibrio cholerae serotype O1 | |
1'-aza-4'-deaza-2'-deoxy-1'-(9-methylene)-immucillin-A | - |
Escherichia coli | |
5'-4-chloro-phenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | - |
Escherichia coli | |
5'-4-chloro-phenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | - |
Salmonella enterica | |
5'-4-chloro-phenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | - |
Vibrio cholerae serotype O1 | |
5'-4-chloro-phenylthio-immucillin-A | - |
Escherichia coli | |
5'-4-chloro-phenylthio-immucillin-A | - |
Salmonella enterica | |
5'-4-chloro-phenylthio-immucillin-A | - |
Vibrio cholerae serotype O1 | |
immucillin-A | - |
Escherichia coli | |
immucillin-A | - |
Salmonella enterica | |
immucillin-A | - |
Vibrio cholerae serotype O1 | |
methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene) -immucillin-A | - |
Salmonella enterica | |
methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | - |
Escherichia coli | |
methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | - |
Vibrio cholerae serotype O1 | |
methylthio-immucillin-A | - |
Escherichia coli | |
methylthio-immucillin-A | - |
Salmonella enterica | |
methylthio-immucillin-A | - |
Vibrio cholerae serotype O1 | |
additional information | inhibitor design and synthesis informed by transition state analysis have developed femtomolar inhibitors, thermodynamic analyses of transition state analogues, overview | Escherichia coli | |
additional information | inhibitor design and synthesis informed by transition state analysis have developed femtomolar inhibitors, thermodynamic analyses of transition state analogues, overview | Salmonella enterica | |
additional information | inhibitor design and synthesis informed by transition state analysis have developed femtomolar inhibitors, thermodynamic analyses of transition state analogues, overview | Vibrio cholerae serotype O1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000019 | - |
S-methyl-5'-thioadenosine | in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C | Escherichia coli | |
0.00000036 | - |
S-methyl-5'-thioadenosine | in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C | Vibrio cholerae serotype O1 | |
0.0000004 | - |
S-methyl-5'-thioadenosine | in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C | Salmonella enterica | |
0.00019 | - |
5'-methylthioadenosine | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
0.00036 | - |
5'-methylthioadenosine | pH 7.5, 25°C, recombinant enzyme | Vibrio cholerae serotype O1 | |
0.0004 | - |
5'-methylthioadenosine | pH 7.5, 25°C, recombinant enzyme | Salmonella enterica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + H2O | Escherichia coli | - |
adenine + S-methyl-5-thio-D-ribose | - |
? | |
5'-methylthioadenosine + H2O | Salmonella enterica | - |
adenine + S-methyl-5-thio-D-ribose | - |
? | |
5'-methylthioadenosine + H2O | Vibrio cholerae serotype O1 | - |
adenine + S-methyl-5-thio-D-ribose | - |
? | |
additional information | Escherichia coli | the enzyme catalyzes the hydrolytic cleavage of adenine from methylthioadenosine | ? | - |
? | |
additional information | Salmonella enterica | the enzyme catalyzes the hydrolytic cleavage of adenine from methylthioadenosine | ? | - |
? | |
additional information | Vibrio cholerae serotype O1 | the enzyme catalyzes the hydrolytic cleavage of adenine from methylthioadenosine | ? | - |
? | |
S-methyl-5'-thioadenosine + H2O | Escherichia coli | - |
S-methyl-5-thio-D-ribose + adenine | - |
? | |
S-methyl-5'-thioadenosine + H2O | Salmonella enterica | - |
S-methyl-5-thio-D-ribose + adenine | - |
? | |
S-methyl-5'-thioadenosine + H2O | Vibrio cholerae serotype O1 | - |
S-methyl-5-thio-D-ribose + adenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Salmonella enterica | - |
- |
- |
Vibrio cholerae serotype O1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Escherichia coli |
Ni-NTA column chromatography | Salmonella enterica |
Ni-NTA column chromatography | Vibrio cholerae serotype O1 |
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21 Star(DE3) by nickel affinity chromatography | Escherichia coli |
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21 Star(DE3) by nickel affinity chromatography | Vibrio cholerae serotype O1 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine | transitions state analogues, overview | Escherichia coli | |
S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine | transitions state analogues, overview | Salmonella enterica | |
S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine | transitions state analogues, overview | Vibrio cholerae serotype O1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + H2O | - |
Escherichia coli | adenine + S-methyl-5-thio-D-ribose | - |
? | |
5'-methylthioadenosine + H2O | - |
Salmonella enterica | adenine + S-methyl-5-thio-D-ribose | - |
? | |
5'-methylthioadenosine + H2O | - |
Vibrio cholerae serotype O1 | adenine + S-methyl-5-thio-D-ribose | - |
? | |
additional information | the enzyme catalyzes the hydrolytic cleavage of adenine from methylthioadenosine | Escherichia coli | ? | - |
? | |
additional information | the enzyme catalyzes the hydrolytic cleavage of adenine from methylthioadenosine | Salmonella enterica | ? | - |
? | |
additional information | the enzyme catalyzes the hydrolytic cleavage of adenine from methylthioadenosine | Vibrio cholerae serotype O1 | ? | - |
? | |
S-methyl-5'-thioadenosine + H2O | - |
Escherichia coli | S-methyl-5-thio-D-ribose + adenine | - |
? | |
S-methyl-5'-thioadenosine + H2O | - |
Salmonella enterica | S-methyl-5-thio-D-ribose + adenine | - |
? | |
S-methyl-5'-thioadenosine + H2O | - |
Vibrio cholerae serotype O1 | S-methyl-5-thio-D-ribose + adenine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Escherichia coli |
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Salmonella enterica |
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Vibrio cholerae serotype O1 |
MTAN | - |
Escherichia coli |
MTAN | - |
Salmonella enterica |
MTAN | - |
Vibrio cholerae serotype O1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
25 | - |
assay at | Salmonella enterica |
25 | - |
assay at | Vibrio cholerae serotype O1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
5'-methylthioadenosine | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
2.3 | - |
S-methyl-5'-thioadenosine | in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C | Escherichia coli | |
4.2 | - |
5'-methylthioadenosine | pH 7.5, 25°C, recombinant enzyme | Vibrio cholerae serotype O1 | |
4.2 | - |
S-methyl-5'-thioadenosine | in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C | Vibrio cholerae serotype O1 | |
20 | - |
5'-methylthioadenosine | pH 7.5, 25°C, recombinant enzyme | Salmonella enterica | |
20 | - |
S-methyl-5'-thioadenosine | in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C | Salmonella enterica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
7.5 | - |
assay at | Salmonella enterica |
7.5 | - |
assay at | Vibrio cholerae serotype O1 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000000004 | - |
5'-4-chloro-phenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C | Escherichia coli | |
0.00000000011 | - |
methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C | Escherichia coli | |
0.0000000027 | - |
5'-4-chloro-phenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C | Salmonella enterica | |
0.000000005 | - |
methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene) -immucillin-A | in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C | Salmonella enterica | |
0.0000000055 | - |
5'-4-chloro-phenylthio-immucillin-A | in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C | Escherichia coli | |
0.0000000093 | - |
5'-4-chloro-phenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C | Vibrio cholerae serotype O1 | |
0.0000000125 | - |
methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C | Vibrio cholerae serotype O1 | |
0.000000038 | - |
5'-4-chloro-phenylthio-immucillin-A | in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C | Salmonella enterica | |
0.000000059 | - |
5'-4-chloro-phenylthio-immucillin-A | in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C | Vibrio cholerae serotype O1 | |
0.000000089 | - |
methylthio-immucillin-A | in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C | Escherichia coli | |
0.000000274 | - |
methylthio-immucillin-A | in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C | Salmonella enterica | |
0.0000003 | - |
methylthio-immucillin-A | in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C | Vibrio cholerae serotype O1 |
General Information | Comment | Organism |
---|---|---|
malfunction | enzyme inhibition disrupts the production of autoinducers, the chemical messengers required for quorum sensing | Escherichia coli |
malfunction | enzyme inhibition disrupts the production of autoinducers, the chemical messengers required for quorum sensing | Salmonella enterica |
malfunction | enzyme inhibition disrupts the production of autoinducers, the chemical messengers required for quorum sensing | Vibrio cholerae serotype O1 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00012 | - |
S-methyl-5'-thioadenosine | in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C | Escherichia coli | |
0.00012 | - |
S-methyl-5'-thioadenosine | in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C | Vibrio cholerae serotype O1 | |
0.5 | - |
5'-methylthioadenosine | pH 7.5, 25°C, recombinant enzyme | Salmonella enterica | |
1.2 | - |
5'-methylthioadenosine | pH 7.5, 25°C, recombinant enzyme | Vibrio cholerae serotype O1 | |
1200 | - |
5'-methylthioadenosine | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
50000 | - |
S-methyl-5'-thioadenosine | in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C | Salmonella enterica |