Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Burkholderia thailandensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
S-methyl-5'-thioadenosine | at pH 7.5 and 25°C | Burkholderia thailandensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, SDS-PAGE | Burkholderia thailandensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Burkholderia thailandensis | the enzyme also shows weak hydrolytic activity with S-adenosylhomocysteine | ? | - |
? | |
S-methyl-5'-thioadenosine + H2O | Burkholderia thailandensis | preferred substrate | S-methyl-5-thio-D-ribose + adenine | - |
? | |
S-methyl-5'-thioadenosine + H2O | Burkholderia thailandensis | almost completely preferred substrate | adenine + S-methyl-5-thio-D-ribose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia thailandensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-bind resin column chromatography | Burkholderia thailandensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme also shows weak hydrolytic activity with S-adenosylhomocysteine | Burkholderia thailandensis | ? | - |
? | |
S-methyl-5'-thioadenosine + H2O | preferred substrate | Burkholderia thailandensis | S-methyl-5-thio-D-ribose + adenine | - |
? | |
S-methyl-5'-thioadenosine + H2O | almost completely preferred substrate | Burkholderia thailandensis | adenine + S-methyl-5-thio-D-ribose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, SDS-PAGE | Burkholderia thailandensis |
Synonyms | Comment | Organism |
---|---|---|
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Burkholderia thailandensis |
MTAN | - |
Burkholderia thailandensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 37 | - |
Burkholderia thailandensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 37 | - |
Burkholderia thailandensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
S-methyl-5'-thioadenosine | at pH 7.5 and 25°C | Burkholderia thailandensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | - |
Burkholderia thailandensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
95 | - |
S-methyl-5'-thioadenosine | at pH 7.5 and 25°C | Burkholderia thailandensis |