Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.2.16 extracted from

  • Wang, S.; Thomas, K.; Schramm, V.L.
    Catalytic site cooperativity in dimeric methylthioadenosine nucleosidase (2014), Biochemistry, 53, 1527-1535.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus
expressed in Escherichia coli BL21(DE3) cells Escherichia coli
expressed in Escherichia coli BL21(DE3) cells Vibrio cholerae

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method Vibrio cholerae
sitting drop vapor diffusion method, using Escherichia coli

Protein Variants

Protein Variants Comment Organism
A113P the mutant shows reduced catalytic efficiency compared to the wild type enzyme Vibrio cholerae
A113P/V153I the mutant shows reduced catalytic efficiency compared to the wild type enzyme Vibrio cholerae
A113P/V153I/R158G the mutant shows reduced catalytic efficiency compared to the wild type enzyme Vibrio cholerae
E173Q inactive Staphylococcus aureus
F104C/C186S the mutant shows reduced activity compared to the wild type Staphylococcus aureus
V153I the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme Vibrio cholerae

Inhibitors

Inhibitors Comment Organism Structure
(3R,4S)-1-[9-deazaadenin-(9-yl)methyl]-3-hydroxy-4-(methylthiomethyl)pyrrolidine
-
 Staphylococcus aureus
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A
-
 Escherichia coli
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A
-
 Vibrio cholerae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000061
-
 S-methyl-5'-thioadenosine wild type enzyme, at pH 7.7 and 25°C Vibrio cholerae
0.000139
-
 S-methyl-5'-thioadenosine wild type enzyme, at pH 7.7 and 25°C Escherichia coli
0.000202
-
 S-methyl-5'-thioadenosine mutant enzyme A113P/V153I/R158G, at pH 7.7 and 25°C Vibrio cholerae
0.000262
-
 S-methyl-5'-thioadenosine mutant enzyme A113P, at pH 7.7 and 25°C Vibrio cholerae
0.000262
-
 S-methyl-5'-thioadenosine mutant enzyme A113P/V153I, at pH 7.7 and 25°C Vibrio cholerae
0.00043
-
 S-methyl-5'-thioadenosine mutant enzyme V153I, at pH 7.7 and 25°C Vibrio cholerae
0.0009
-
 S-methyl-5'-thioadenosine wild type enzyme, at pH 7.2 and 25°C Staphylococcus aureus
0.0029
-
 S-methyl-5'-thioadenosine mutant enzyme F104C/C186S, at pH 7.2 and 25°C Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-homocysteine + H2O Staphylococcus aureus
-
 S-D-ribosyl-L-homocysteine + adenine
-
 ir
S-methyl-5'-thioadenosine + H2O Staphylococcus aureus
-
 S-methyl-5-thio-D-ribose + adenine
-
 ?
S-methyl-5'-thioadenosine + H2O Escherichia coli
-
 S-methyl-5-thio-D-ribose + adenine
-
 ?
S-methyl-5'-thioadenosine + H2O Vibrio cholerae
-
 S-methyl-5-thio-D-ribose + adenine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AF12
-
-
Staphylococcus aureus Q99TQ0
-
-
Vibrio cholerae A5F5R2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Escherichia coli
Ni-NTA column chromatography Vibrio cholerae
Ni-NTA column chromatography and Superdex 200 gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-homocysteine + H2O
-
 Staphylococcus aureus S-D-ribosyl-L-homocysteine + adenine
-
 ir
S-methyl-5'-thioadenosine + H2O
-
 Staphylococcus aureus S-methyl-5-thio-D-ribose + adenine
-
 ?
S-methyl-5'-thioadenosine + H2O
-
 Escherichia coli S-methyl-5-thio-D-ribose + adenine
-
 ?
S-methyl-5'-thioadenosine + H2O
-
 Vibrio cholerae S-methyl-5-thio-D-ribose + adenine
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Staphylococcus aureus

Synonyms

Synonyms Comment Organism
5'-methylthioadenosine/S-adenosyl-L-homocysteine nucleosidase
-
 Escherichia coli
5'-methylthioadenosine/S-adenosyl-L-homocysteine nucleosidase
-
 Vibrio cholerae
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
-
 Staphylococcus aureus
methylthioadenosine nucleosidase
-
 Escherichia coli
methylthioadenosine nucleosidase
-
 Vibrio cholerae
MTAN
-
 Staphylococcus aureus
MTAN
-
 Escherichia coli
MTAN
-
 Vibrio cholerae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0079
-
 S-methyl-5'-thioadenosine mutant enzyme V153I, at pH 7.7 and 25°C Vibrio cholerae
0.074
-
 S-methyl-5'-thioadenosine mutant enzyme A113P/V153I, at pH 7.7 and 25°C Vibrio cholerae
0.11
-
 S-methyl-5'-thioadenosine mutant enzyme A113P/V153I/R158G, at pH 7.7 and 25°C Vibrio cholerae
0.17
-
 S-methyl-5'-thioadenosine mutant enzyme A113P, at pH 7.7 and 25°C Vibrio cholerae
0.18
-
 S-methyl-5'-thioadenosine wild type enzyme, at pH 7.7 and 25°C Vibrio cholerae
0.32
-
 S-methyl-5'-thioadenosine wild type enzyme, at pH 7.7 and 25°C Escherichia coli
7.2
-
 S-methyl-5'-thioadenosine mutant enzyme F104C/C186S, at pH 7.2 and 25°C Staphylococcus aureus
10.2
-
 S-methyl-5'-thioadenosine wild type enzyme, at pH 7.2 and 25°C Staphylococcus aureus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
18
-
 S-methyl-5'-thioadenosine mutant enzyme V153I, at pH 7.7 and 25°C Vibrio cholerae
200
-
 S-methyl-5'-thioadenosine mutant enzyme A113P/V153I/R158G, at pH 7.7 and 25°C Vibrio cholerae
400
-
 S-methyl-5'-thioadenosine mutant enzyme A113P/V153I, at pH 7.7 and 25°C Vibrio cholerae
700
-
 S-methyl-5'-thioadenosine mutant enzyme A113P, at pH 7.7 and 25°C Vibrio cholerae
2300
-
 S-methyl-5'-thioadenosine wild type enzyme, at pH 7.7 and 25°C Escherichia coli
2480
-
 S-methyl-5'-thioadenosine mutant enzyme F104C/C186S, at pH 7.2 and 25°C Staphylococcus aureus
3000
-
 S-methyl-5'-thioadenosine wild type enzyme, at pH 7.7 and 25°C Vibrio cholerae
11300
-
 S-methyl-5'-thioadenosine wild type enzyme, at pH 7.2 and 25°C Staphylococcus aureus