Cloned (Comment) | Organism |
---|---|
isozyme AtMTAN1, overexpression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
overexpression of wild-type and mutant His-tagged enzyme in strain BL21(DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
crystal structure comparison of MTAN from Escherichia coli with the isozyme AtMTAN1 from Arabidopsis thaliana, overview | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
E174Q | site-directed mutagenesis, structure comparison with the wild-type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and thermodynamics, overview | Escherichia coli | |
additional information | - |
additional information | kinetics and thermodynamics, overview | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | AtMTAN2 is dual-substrate specific and is responsible for the hydrolysis of 5'-methylthioadenosine and S-adenosylhomocysteine to adenine and their corresponding thioriboses, overview | ? | - |
? | |
additional information | Escherichia coli | MTAN is dual-substrate specific and is responsible for the hydrolysis of 5'-methylthioadenosine and S-adenosylhomocysteine to adenine and their corresponding thioriboses, overview | ? | - |
? | |
S-methyl-5'-thioadenosine + H2O | Escherichia coli | - |
S-methyl-5-thio-D-ribose + adenine | - |
? | |
S-methyl-5'-thioadenosine + H2O | Arabidopsis thaliana | - |
S-methyl-5-thio-D-ribose + adenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
isozyme AtMTAN1 | - |
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozyme AtMTAN1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Arabidopsis thaliana |
recombinant wild-type and mutant His-tagged MTAN from strain BL21(DE3) by nickel affinity chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
amide proton exchange rates of isozyme AtMTAN1 | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | AtMTAN2 is dual-substrate specific and is responsible for the hydrolysis of 5'-methylthioadenosine and S-adenosylhomocysteine to adenine and their corresponding thioriboses, overview | Arabidopsis thaliana | ? | - |
? | |
additional information | MTAN is dual-substrate specific and is responsible for the hydrolysis of 5'-methylthioadenosine and S-adenosylhomocysteine to adenine and their corresponding thioriboses, overview | Escherichia coli | ? | - |
? | |
additional information | dynamics are an important factor in substrate selection in MTAN, overview. Substrate binding induces a favourable entropic change in AtMTAN1 | Arabidopsis thaliana | ? | - |
? | |
additional information | substrate binding, specificity and dynamics, overview. Substrate binding induces a unfavourable entropic change | Escherichia coli | ? | - |
? | |
S-methyl-5'-thioadenosine + H2O | - |
Escherichia coli | S-methyl-5-thio-D-ribose + adenine | - |
? | |
S-methyl-5'-thioadenosine + H2O | - |
Arabidopsis thaliana | S-methyl-5-thio-D-ribose + adenine | - |
? | |
S-methyl-5'-thioadenosine + H2O | preferred substrate | Arabidopsis thaliana | S-methyl-5-thio-D-ribose + adenine | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure comparison of isozyme AtMTAN1 with the MTAN from Escherichia coli, overview | Arabidopsis thaliana |
More | structure comparison of MTAN from Escherichia coli with isozyme AtMTAN from Arabidopsis thaliana, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
5-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Escherichia coli |
5-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Arabidopsis thaliana |
MTA/SAH nucleosidase | - |
Escherichia coli |
MTA/SAH nucleosidase | - |
Arabidopsis thaliana |
MTAN | - |
Escherichia coli |
MTAN | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
7.5 | - |
assay at | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
metabolism | 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase plays a key role in the methionine-recycling pathway of bacteria and plants | Escherichia coli |
metabolism | 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase plays a key role in the methionine-recycling pathway of bacteria and plants | Arabidopsis thaliana |