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Literature summary for 3.2.2.16 extracted from

  • Gutierrez, J.A.; Crowder, T.; Rinaldo-Matthis, A.; Ho, M.C.; Almo, S.C.; Schramm, V.L.
    Transition state analogs of 5'-methylthioadenosine nucleosidase disrupt quorum sensing (2009), Nat. Chem. Biol., 5, 251-257.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as His-tagged protein Vibrio cholerae serotype O1

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with But-dadme-imma A, sitting drop vapour diffusion method, with 0.2 M potassium iodide and 20% (w/v) PEG3350 Vibrio cholerae serotype O1

Inhibitors

Inhibitors Comment Organism Structure
benzylthio-immucillin A
-
 Vibrio cholerae serotype O1
butylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A picomolar inhibitor of MTAN Vibrio cholerae serotype O1
ethylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A picomolar inhibitor of MTAN Vibrio cholerae serotype O1
ethylthio-immucillin A
-
 Vibrio cholerae serotype O1
methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A picomolar inhibitor of MTAN Vibrio cholerae serotype O1
methylthio-immucillin A
-
 Vibrio cholerae serotype O1
p-chlorophenylthio-immucillin A
-
 Vibrio cholerae serotype O1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 5'-methylthioadenosine at 25°C, in 100 mM HEPES, pH 7.5, and 50 mM KCl Vibrio cholerae serotype O1

Organism

Organism UniProt Comment Textmining
Vibrio cholerae serotype O1
-
-
-
Vibrio cholerae serotype O1 N16961
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-methylthioadenosine + H2O
-
 Vibrio cholerae serotype O1 5-methylthio-D-ribose + adenine
-
 ?
5'-methylthioadenosine + H2O
-
 Vibrio cholerae serotype O1 N16961 5-methylthio-D-ribose + adenine
-
 ?

Synonyms

Synonyms Comment Organism
5'-methylthioadenosine nucleosidase
-
 Vibrio cholerae serotype O1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
 5'-methylthioadenosine at 25°C, in 100 mM HEPES, pH 7.5, and 50 mM KCl Vibrio cholerae serotype O1

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000007
-
 ethylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A at 25°C, in 50 mM phosphate buffer, pH 7.9, and 10 mM KCl Vibrio cholerae serotype O1
0.000000073
-
 methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A at 25°C, in 50 mM phosphate buffer, pH 7.9, and 10 mM KCl Vibrio cholerae serotype O1
0.000000208
-
 butylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A at 25°C, in 50 mM phosphate buffer, pH 7.9, and 10 mM KCl Vibrio cholerae serotype O1

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000006
-
 at 25°C, in 50 mM phosphate buffer, pH 7.9, and 10 mM KCl Vibrio cholerae serotype O1 butylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A
0.000027
-
 at 25°C, in 50 mM phosphate buffer, pH 7.9, and 10 mM KCl Vibrio cholerae serotype O1 methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A
0.000031
-
 at 25°C, in 50 mM phosphate buffer, pH 7.9, and 10 mM KCl Vibrio cholerae serotype O1 ethylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A