Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
in complex with the transition-state analogue formycin A, hanging drop vapour diffusion method, using 50 mM potassium phosphate with 20% (w/v) PEG 8000 | Staphylococcus aureus |
in complex with the transition-state analogue formycin A, hanging drop vapour diffusion method, using 50 mM potassium phosphate with 20% (w/v) PEG 8000 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00124 | - |
5'-methylthioadenosine | recombinant enzyme, in 50 mM potassium phosphate, pH 7.0 | Staphylococcus aureus | |
0.00738 | - |
5'-methylthioadenosine | recombinant enzyme, in 50 mM potassium phosphate, pH 7.0 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AF14 | - |
- |
Staphylococcus aureus | Q99TQ0 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA Superflow resin column chromatography and Superdex-200 gel filtration | Staphylococcus aureus |
Ni-NTA Superflow resin column chromatography and Superdex-200 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + H2O | - |
Staphylococcus aureus | 5-methylthio-D-ribose + adenine | - |
? | |
5'-methylthioadenosine + H2O | - |
Escherichia coli | 5-methylthio-D-ribose + adenine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | biologically active dimer, X-ray crystallography | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
5-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Staphylococcus aureus |
5-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Escherichia coli |
MTAN | MTAN is a dual substrate-specific enzyme that irreversibly hydrolyzes the glycosidic bond of 5'-methylthioadenosine or S-adenosyl-L-homocysteine | Staphylococcus aureus |
MTAN | MTAN is a dual substrate-specific enzyme that irreversibly hydrolyzes the glycosidic bond of 5'-methylthioadenosine or S-adenosyl-L-homocysteine | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00973 | - |
5'-methylthioadenosine | recombinant enzyme, in 50 mM potassium phosphate, pH 7.0 | Staphylococcus aureus | |
0.49 | - |
5'-methylthioadenosine | recombinant enzyme, in 50 mM potassium phosphate, pH 7.0 | Escherichia coli |