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Literature summary for 3.2.2.16 extracted from

  • Singh, V.; Shi, W.; Almo, S.C.; Evans, G.B.; Furneaux, R.H.; Tyler, P.C.; Painter, G.F.; Lenz, D.H.; Mee, S.; Zheng, R.; Schramm, V.L.
    Structure and inhibition of a quorum sensing target from Streptococcus pneumoniae (2006), Biochemistry, 45, 12929-12941.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.16

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Escherichia coli
expression in Escherichia coli Streptococcus pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in complex with transition state analogue methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A. Enzyme forms a dimer with the methylthio group in a flexible hydrophobic pocket Escherichia coli
enzyme in complex with transition state analogue methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A. Enzyme forms a dimer with the methylthio group in a flexible hydrophobic pocket Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
4-chlorophenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A
-
 Escherichia coli
4-chlorophenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A
-
 Streptococcus pneumoniae
ethylthio-immucillin-A
-
 Escherichia coli
ethylthio-immucillin-A
-
 Streptococcus pneumoniae
methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A
-
 Escherichia coli
methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A
-
 Streptococcus pneumoniae
methylthio-immucillin-A weak Escherichia coli
methylthio-immucillin-A weak Streptococcus pneumoniae
p-tolylthio-immucillin-A
-
 Escherichia coli
p-tolylthio-immucillin-A
-
 Streptococcus pneumoniae
phenylthio-immucillin-A
-
 Escherichia coli
phenylthio-immucillin-A
-
 Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 S-adenosyl-L-homocysteine pH 7.5, 25°C Streptococcus pneumoniae
0.023
-
 5'-methylthioadenosine pH 7.5, 25°C Streptococcus pneumoniae
0.43
-
 5'-methylthioadenosine pH 7.5, 25°C Escherichia coli
1.3
-
 S-adenosyl-L-homocysteine pH 7.5, 25°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 bifunctional 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
-
Streptococcus pneumoniae Q8DQ16 bifunctional 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-methylthioadenosine + H2O
-
 Escherichia coli 5-methylthio-D-ribose + adenine
-
 ?
5'-methylthioadenosine + H2O
-
 Streptococcus pneumoniae 5-methylthio-D-ribose + adenine
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Escherichia coli adenine + S-ribosyl-L-homocysteine
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Streptococcus pneumoniae adenine + S-ribosyl-L-homocysteine
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.25
-
 5'-methylthioadenosine pH 7.5, 25°C Streptococcus pneumoniae
0.37
-
 S-adenosyl-L-homocysteine pH 7.5, 25°C Streptococcus pneumoniae
2.1
-
 S-adenosyl-L-homocysteine pH 7.5, 25°C Escherichia coli
4
-
 5'-methylthioadenosine pH 7.5, 25°C Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000036
-
 4-chlorophenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A pH 7.5, 25°C Escherichia coli
0.00000036
-
 4-chlorophenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A pH 7.5, 25°C Streptococcus pneumoniae
0.000024
-
 methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A pH 7.5, 25°C Escherichia coli
0.000024
-
 methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A pH 7.5, 25°C Streptococcus pneumoniae
0.00004
-
 ethylthio-immucillin-A pH 7.5, 25°C Escherichia coli
0.00004
-
 ethylthio-immucillin-A pH 7.5, 25°C Streptococcus pneumoniae
0.00006
-
 p-tolylthio-immucillin-A pH 7.5, 25°C Escherichia coli
0.00006
-
 p-tolylthio-immucillin-A pH 7.5, 25°C Streptococcus pneumoniae
0.000335
-
 phenylthio-immucillin-A pH 7.5, 25°C Escherichia coli
0.000335
-
 phenylthio-immucillin-A pH 7.5, 25°C Streptococcus pneumoniae
0.001
-
 methylthio-immucillin-A pH 7.5, 25°C Escherichia coli
0.001
-
 methylthio-immucillin-A pH 7.5, 25°C Streptococcus pneumoniae