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Literature summary for 3.2.2.14 extracted from

  • Imai, T.
    Properties of allosteric nicotinamide mononucleotide glycohydrolase from Azotobacter vinelandii: Activation and inhibition (1987), J. Biochem., 101, 163-173.
    View publication on PubMed
Search for more literature for 3.2.2.14

Activating Compound

Activating Compound Comment Organism Structure
guanosine 3'-diphosphate-5'-triphosphate guanine nucleotides, guanosinetriphosphate and polyphosphate derivatives, effective activation Azotobacter vinelandii
RNA
-
 Azotobacter vinelandii
SO42- 10 mM relative activity 103%, 100% activity with nicotinamide as substrate Azotobacter vinelandii

Inhibitors

Inhibitors Comment Organism Structure
5-phosphoribose 1-diphosphate PRPP, inorganic polyphosphates, affinity of 5-phosphoribose 1-pyrophosphate for the enzyme is dependent on the activator concentration , inhibition mechanism with 5-phosphoribose 1-pyrophosphate is competitive with GTP Azotobacter vinelandii
7-methylguanosine-5'-triphosphate
-
 Azotobacter vinelandii
ATP
-
 Azotobacter vinelandii
CTP
-
 Azotobacter vinelandii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0087
-
 guanosine 3'-diphosphate 5'-triphosphate
-
 Azotobacter vinelandii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 1 * 110000 + 1 * 110000, enzyme easily dissociates in presence of 1 mM ATP, consisting of 4 identical protomers, MW 55000, no dissociation in presence of GTP Azotobacter vinelandii
210000
-
 gel filtration Azotobacter vinelandii
240000
-
-
 Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nicotinamide mononucleotide + H2O Azotobacter vinelandii biological function reusing NMN resulting from degradation of pyridine cofactors by nucleotide pyrophosphatase, EC 3.6.1.9, in concert with NMN aminohydrolase, EC 3.5.1.42 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Azotobacter vinelandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanosine 3'-diphosphate 5'-triphosphate + H2O
-
 Azotobacter vinelandii ?
-
 ?
nicotinamide mononucleotide + H2O biological function reusing NMN resulting from degradation of pyridine cofactors by nucleotide pyrophosphatase, EC 3.6.1.9, in concert with NMN aminohydrolase, EC 3.5.1.42 Azotobacter vinelandii ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 110000 + 1 * 110000, enzyme easily dissociates in presence of 1 mM ATP, consisting of 4 identical protomers, MW 55000, no dissociation in presence of GTP Azotobacter vinelandii