Cloned (Comment) | Organism |
---|---|
- |
Caenorhabditis elegans |
Crystallization (Comment) | Organism |
---|---|
to 1.65 A resolution. Both residues Cys 253 and Cys 42 are appropriately positioned to interact with the purine ring of the substrate | Caenorhabditis elegans |
Protein Variants | Comment | Organism |
---|---|---|
C253A | kcat/KM values for the hydrolysis of inosine, adenosine and guanosine are reduced by a factor of about 120fold compared to the wild-tpe enzyme | Caenorhabditis elegans |
C42A | the kcat/KM values for the hydrolysis of inosine, adenosine, and guanosine are reduced by a factor of about 14-, 22-, and 10fold, respectively, compared to the wild-type enzyme | Caenorhabditis elegans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00032 | - |
4-nitrophenylriboside | wild-type, pH 7.5, 25°C | Caenorhabditis elegans | |
0.00064 | - |
4-nitrophenylriboside | mutant C42A, pH 7.5, 25°C | Caenorhabditis elegans | |
0.009 | - |
4-nitrophenylriboside | mutant C253A, ApH 7.5, 25°C | Caenorhabditis elegans | |
0.037 | - |
7-methylguanosine | wild-type, pH 7.5, 25°C | Caenorhabditis elegans | |
0.051 | - |
7-methylguanosine | mutant C253A, ApH 7.5, 25°C | Caenorhabditis elegans | |
0.053 | - |
7-methylguanosine | mutant C42A, pH 7.5, 25°C | Caenorhabditis elegans | |
0.054 | - |
guanosine | mutant C42A, pH 7.5, 25°C | Caenorhabditis elegans | |
0.068 | - |
guanosine | wild-type, pH 7.5, 25°C | Caenorhabditis elegans | |
0.077 | - |
adenosine | wild-type, pH 7.5, 25°C | Caenorhabditis elegans | |
0.094 | - |
adenosine | mutant C42A, pH 7.5, 25°C | Caenorhabditis elegans | |
0.096 | - |
adenosine | mutant C253A, ApH 7.5, 25°C | Caenorhabditis elegans | |
0.129 | - |
Inosine | wild-type, pH 7.5, 25°C | Caenorhabditis elegans | |
0.215 | - |
Inosine | mutant C42A, pH 7.5, 25°C | Caenorhabditis elegans | |
0.3 | - |
guanosine | mutant C253A, ApH 7.5, 25°C | Caenorhabditis elegans | |
0.343 | - |
Inosine | mutant C253A, ApH 7.5, 25°C | Caenorhabditis elegans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | Q9XWN7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Caenorhabditis elegans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenylriboside + H2O | - |
Caenorhabditis elegans | D-ribose + 4-nitrophenol | - |
? | |
7-methylguanosine + H2O | - |
Caenorhabditis elegans | D-ribose + 7-methylguanine | - |
? | |
adenosine + H2O | - |
Caenorhabditis elegans | D-ribose + adenine | - |
? | |
guanosine + H2O | - |
Caenorhabditis elegans | D-ribose + guanine | - |
? | |
inosine + H2O | - |
Caenorhabditis elegans | D-ribose + hypoxanthine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CELE_Y43F8C.13 | - |
Caenorhabditis elegans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0042 | - |
adenosine | mutant C253A, ApH 7.5, 25°C | Caenorhabditis elegans | |
0.0043 | - |
Inosine | mutant C253A, ApH 7.5, 25°C | Caenorhabditis elegans | |
0.0051 | - |
guanosine | mutant C253A, ApH 7.5, 25°C | Caenorhabditis elegans | |
0.011 | - |
guanosine | mutant C42A, pH 7.5, 25°C | Caenorhabditis elegans | |
0.017 | - |
4-nitrophenylriboside | mutant C253A, ApH 7.5, 25°C | Caenorhabditis elegans | |
0.023 | - |
adenosine | mutant C42A, pH 7.5, 25°C | Caenorhabditis elegans | |
0.024 | - |
Inosine | mutant C42A, pH 7.5, 25°C | Caenorhabditis elegans | |
0.026 | - |
4-nitrophenylriboside | mutant C42A, pH 7.5, 25°C | Caenorhabditis elegans | |
0.039 | - |
4-nitrophenylriboside | wild-type, pH 7.5, 25°C | Caenorhabditis elegans | |
0.143 | - |
guanosine | wild-type, pH 7.5, 25°C | Caenorhabditis elegans | |
0.203 | - |
Inosine | wild-type, pH 7.5, 25°C | Caenorhabditis elegans | |
0.418 | - |
adenosine | wild-type, pH 7.5, 25°C | Caenorhabditis elegans | |
1.09 | - |
7-methylguanosine | wild-type, pH 7.5, 25°C | Caenorhabditis elegans | |
1.98 | - |
7-methylguanosine | mutant C253A, ApH 7.5, 25°C | Caenorhabditis elegans | |
2.4 | - |
7-methylguanosine | mutant C42A, pH 7.5, 25°C | Caenorhabditis elegans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
Inosine | mutant C253A, ApH 7.5, 25°C | Caenorhabditis elegans | |
0.017 | - |
guanosine | mutant C253A, ApH 7.5, 25°C | Caenorhabditis elegans | |
0.044 | - |
adenosine | mutant C253A, ApH 7.5, 25°C | Caenorhabditis elegans | |
0.112 | - |
Inosine | mutant C42A, pH 7.5, 25°C | Caenorhabditis elegans | |
0.202 | - |
guanosine | mutant C42A, pH 7.5, 25°C | Caenorhabditis elegans | |
0.245 | - |
adenosine | mutant C42A, pH 7.5, 25°C | Caenorhabditis elegans | |
1.574 | - |
Inosine | wild-type, pH 7.5, 25°C | Caenorhabditis elegans | |
1.89 | - |
4-nitrophenylriboside | mutant C253A, ApH 7.5, 25°C | Caenorhabditis elegans | |
2.103 | - |
guanosine | wild-type, pH 7.5, 25°C | Caenorhabditis elegans | |
5.429 | - |
adenosine | wild-type, pH 7.5, 25°C | Caenorhabditis elegans | |
29.46 | - |
7-methylguanosine | wild-type, pH 7.5, 25°C | Caenorhabditis elegans | |
38.82 | - |
7-methylguanosine | mutant C253A, ApH 7.5, 25°C | Caenorhabditis elegans | |
40.63 | - |
4-nitrophenylriboside | mutant C42A, pH 7.5, 25°C | Caenorhabditis elegans | |
45.28 | - |
7-methylguanosine | mutant C42A, pH 7.5, 25°C | Caenorhabditis elegans | |
121.88 | - |
4-nitrophenylriboside | wild-type, pH 7.5, 25°C | Caenorhabditis elegans |