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Literature summary for 3.2.2.1 extracted from

  • Liang, L.; He, X.; Liu, G.; Tan, H.
    The role of a purine-specific nucleoside hydrolase in spore germination of Bacillus thuringiensis (2008), Microbiology, 154, 1333-1340.
    View publication on PubMed
Search for more literature for 3.2.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus thuringiensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.399
-
 Inosine in 50 mM HEPES (pH 7.3), at 25°C Bacillus thuringiensis

Localization

Localization Comment Organism GeneOntology No. Textmining
exosporium
-
 Bacillus thuringiensis 43592
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36200
-
 calculated from amino acid sequence Bacillus thuringiensis

Organism

Organism UniProt Comment Textmining
Bacillus thuringiensis A7UHH1 subspecies Bacillus thuringiensis kurstaki
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Bacillus thuringiensis

Source Tissue

Source Tissue Comment Organism Textmining
spore
-
 Bacillus thuringiensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.32
-
 in 50 mM HEPES, pH 7.3, with guanosine as substrate Bacillus thuringiensis
11.76
-
 in 50 mM HEPES, pH 7.3, with adenosine as substrate Bacillus thuringiensis
57.2
-
 in 50 mM HEPES, pH 7.3, with inosine as substrate Bacillus thuringiensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine + H2O
-
 Bacillus thuringiensis D-ribose + adenine
-
 ?
guanosine + H2O
-
 Bacillus thuringiensis D-ribose + guanine
-
 ?
inosine + H2O
-
 Bacillus thuringiensis D-ribose + hypoxanthine
-
 ?
additional information hydrolase activity could not be detected when uridine or cytidine is used as a substrate Bacillus thuringiensis ?
-
 ?
additional information IunH can hydrolyse inosine, adenosine and guanosine with the activity order: inosine Bacillus thuringiensis ?
-
 ?

Synonyms

Synonyms Comment Organism
inosine/adenosine/guanosinepreferring NH
-
 Bacillus thuringiensis
IunH IunH is important in moderating inosine- or adenosine-induced germination of Bacillus thuringiensis spores Bacillus thuringiensis
purine-specific NH
-
 Bacillus thuringiensis
purine-specific nucleoside hydrolase
-
 Bacillus thuringiensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Bacillus thuringiensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 100 the activity is stable over a wide temperature range, the highest activity being at 80°C with high activity being maintained up to 100°C Bacillus thuringiensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
48.9
-
 Inosine in 50 mM HEPES (pH 7.3), at 25°C Bacillus thuringiensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bacillus thuringiensis

pH Range

pH Minimum pH Maximum Comment Organism
5 10 inosine hydrolase activity of the purified recombinant IunH is detected over a wide range of pH values, the highest activity being obtained at pH 6 Bacillus thuringiensis