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Literature summary for 3.2.2.1 extracted from

  • Versees, W.; Goeminne, A.; Berg, M.; Vandemeulebroucke, A.; Haemers, A.; Augustyns, K.; Steyaert, J.
    Crystal structures of T. vivax nucleoside hydrolase in complex with new potent and specific inhibitors (2009), Biochim. Biophys. Acta, 1794, 953-960.
    View publication on PubMed
Search for more literature for 3.2.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli WK6 cells Trypanosoma vivax

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with inhibitors, hanging drop vapour diffusion method, using 25% (w/v) PEG 3350 in 100 mM Bis-Tris buffer pH 6.5 and 0.2 M MgCl2 Trypanosoma vivax

Inhibitors

Inhibitors Comment Organism Structure
(2R,3R,4S)-1-[(4-hydroxy-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-2-(hydroxymethyl)pyrrolidin-3,4-diol nanomolar inhibitor Trypanosoma vivax
(2R,3R,4S)-2-(hydroxymethyl)-1-(quinolin-8-ylmethyl)pyrrolidin-3,4-diol nanomolar inhibitor Trypanosoma vivax
(2S,3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-2-(hydroxymethyl)pyrrolidine-3,4-diol nanomolar inhibitor Trypanosoma vivax
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin H
-
 Trypanosoma vivax
immucillin H immucilin H is (1S)-1-(9-deazahypoxanthin-9-yl)-1,4-dideoxy-1,4-imino-D-ribitol Trypanosoma vivax

Organism

Organism UniProt Comment Textmining
Trypanosoma vivax Q9GPQ4
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex-200 gel filtration Trypanosoma vivax

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine + H2O
-
 Trypanosoma vivax D-ribose + adenine
-
 ?

Synonyms

Synonyms Comment Organism
IAG-NH
-
 Trypanosoma vivax
inosine-adenosine-guanosine-preferring nucleoside hydrolase
-
 Trypanosoma vivax
purine-specific nucleoside hydrolase
-
 Trypanosoma vivax

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000041
-
 (2S,3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-2-(hydroxymethyl)pyrrolidine-3,4-diol
-
 Trypanosoma vivax
0.0000044
-
 (2R,3R,4S)-1-[(4-hydroxy-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-2-(hydroxymethyl)pyrrolidin-3,4-diol
-
 Trypanosoma vivax
0.0000062
-
 immucillin H
-
 Trypanosoma vivax
0.0000108
-
 (2R,3R,4S)-2-(hydroxymethyl)-1-(quinolin-8-ylmethyl)pyrrolidin-3,4-diol
-
 Trypanosoma vivax