Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Saccharolobus solfataricus |
General Stability | Organism |
---|---|
IAG-NH is characterized by remarkable resistance to guanidinium chloride-induced unfolding | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | after 1 h incubation with EDTA (5 mM) the enzyme activity is reduced to less than 0.05% and is restored by addition of 20 mM CaCl2 | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
adenosine | at 80°C | Saccharolobus solfataricus | |
0.16 | - |
guanosine | at 80°C | Saccharolobus solfataricus | |
0.36 | - |
Inosine | at 80°C | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | IAG-NH is a Ca2+-dependent enzyme | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34325 | - |
2 * 34325, calculated from sequence of cDNA | Saccharolobus solfataricus |
35000 | - |
subunit, SDS-PAGE | Saccharolobus solfataricus |
68650 | - |
calculated from sequence of cDNA | Saccharolobus solfataricus |
Organic Solvent | Comment | Organism |
---|---|---|
dithiothreitol | after 1 h pre-incubation at temperatures until 80°C, the enzyme remains completely stable even at high concentrations of dithiothreitol (0.7 M) whereas it becomes susceptible to the effect of the reducing agent as the temperature raises, after 1 h pre-incubation in the presence of 0.5 M, 0.6 M and 0.7 M dithiothreitol, IAG-NH retains 70%, 60% and 21% activity respectively | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus P2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
methylthioinosine-Sepharose column chromatography | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine + H2O | IAG-NH shows higher affinity for adenosine with respect to inosine and guanosine, but the catalytic efficiency of these substrates is comparable | Saccharolobus solfataricus | D-ribose + adenine | - |
? | |
guanosine + H2O | highest activity | Saccharolobus solfataricus | D-ribose + guanine | - |
? | |
guanosine + H2O | highest activity | Saccharolobus solfataricus P2 | D-ribose + guanine | - |
? | |
inosine + H2O | - |
Saccharolobus solfataricus | D-ribose + hypoxanthine | - |
? | |
additional information | IAG-NH does not recognize as substrate nucleosides 5'-phosphates, NAD+ and NADP+ | Saccharolobus solfataricus | ? | - |
? | |
additional information | IAG-NH does not recognize as substrate nucleosides 5'-phosphates, NAD+ and NADP+ | Saccharolobus solfataricus P2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 34325, calculated from sequence of cDNA | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
IAG-NH | the enzyme is specific for adenosine, guanosine and inosine | Saccharolobus solfataricus |
inosine-adenosine-guanosine nucleoside hydrolase | - |
Saccharolobus solfataricus |
purine-specific ribonucleoside hydrolase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
105 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 115 | the enzyme is highly thermoactive, its activity increases sharply up to the optimal temperature of 105°C and 50% activity is still observed at 115°C | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | 110 | IAG-NH is characterized by extreme thermodynamic stability with a melting temperature at 107°C, it retains full activity after 1 h incubation at 90°C and shows half-lives of 45 min, 30 min and 15 min at 100°C, 105°C, and 110°C, respectively | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.8 | - |
adenosine | at 80°C | Saccharolobus solfataricus | |
23.3 | - |
Inosine | at 80°C | Saccharolobus solfataricus | |
29.1 | - |
guanosine | at 80°C | Saccharolobus solfataricus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharolobus solfataricus | estimated isoelectric point | - |
5.9 |