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Literature summary for 3.2.2.1 extracted from

  • Abu-Shady, M.R.; Elshafei, A.M.; El-Beih, F.M.; Mohamed, L.A.
    Hydrolytic cleavage of purine ribunucleosides by extracts of Aspergillus terricola (1994), Acta Microbiol. Pol., 43, 297-304.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4
-
Aspergillus terricola
CoSO4
-
Aspergillus terricola
CuSO4
-
Aspergillus terricola
KCl slight inhibition Aspergillus terricola
Li2SO4 inhibits activity with adenosine and guanosine, slight inhibition of activity with inosine Aspergillus terricola
MnSO4 inhibits activity with adenosine and guanosine, very slight inhibition of activity with inosine Aspergillus terricola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10
-
Inosine
-
Aspergillus terricola
20
-
adenosine
-
Aspergillus terricola
22.2
-
guanosine
-
Aspergillus terricola

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 slight activation Aspergillus terricola
MnCl2 slight activation Aspergillus terricola

Organism

Organism UniProt Comment Textmining
Aspergillus terricola
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine + H2O
-
Aspergillus terricola D-ribose + adenine
-
?
guanosine + H2O
-
Aspergillus terricola D-ribose + guanine
-
?
inosine + H2O
-
Aspergillus terricola hypoxanthine + D-ribose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Aspergillus terricola

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 40°C: about 55% of maximal activity with inosine, about 45% of maximal activity with guanosine, about 40% of maximal activity with adenosine, 70°C: about 30% of maximal activity with inosine, about 35% of maximal activity with guanosine, about 25% of maximal activity with adenosine Aspergillus terricola

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
15 min in absence of substrate, 54-86% loss of activity Aspergillus terricola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Aspergillus terricola