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Literature summary for 3.2.2.1 extracted from

  • Kuwahara, M.; Fujii, T.
    Purification and some properties of NAD-degrading purine nucleosidase from Aspergillus niger (1978), Can. J. Biochem., 56, 345-348.
    View publication on PubMed
Search for more literature for 3.2.2.1

Inhibitors

Inhibitors Comment Organism Structure
HgCl2 10 mM, 17% inhibition Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6
-
 Inosine
-
 Aspergillus niger
2.9
-
 5'-AMP
-
 Aspergillus niger
3
-
 NAD+
-
 Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Aspergillus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
55
-
-
 Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O
-
 Aspergillus niger adenine + D-ribose 5-phosphate
-
 ?
5'-GMP + H2O
-
 Aspergillus niger guanine + D-ribose 5-phosphate
-
 ?
5'-IMP + H2O
-
 Aspergillus niger hypoxanthine + D-ribose 5-phosphate
-
 ?
adenosine + H2O
-
 Aspergillus niger D-ribose + adenine
-
 ?
deamino-NAD+ + H2O
-
 Aspergillus niger ?
-
 ?
guanosine + H2O
-
 Aspergillus niger D-ribose + guanine
-
 ?
inosine + H2O
-
 Aspergillus niger hypoxanthine + D-ribose
-
 ?
NAD+ + H2O
-
 Aspergillus niger adenine + ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 hydrolysis of 3'-AMP Aspergillus niger
4 4.5 degradation of NAD+ Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 90 min, 37°C Aspergillus niger