Any feedback?
Literature summary for 3.2.1.B8 extracted from
- A novel amylolytic enzyme from Thermotoga maritima, resembling cyclodextrinase and alpha-glucosidase, that liberates glucose from the reducing end of the substrates (2002), Biochem. Biophys. Res. Commun., 295, 818-825.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene TM1835, expression in Escherichia coli strain MC1061 | Thermotoga maritima MSB8 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Thermotoga maritima MSB8 |  |
| Co2+ | - |
Thermotoga maritima MSB8 | |
| Fe2+ | - |
Thermotoga maritima MSB8 | |
| Mg2+ | - |
Thermotoga maritima MSB8 | |
| Mn2+ | - |
Thermotoga maritima MSB8 | |
| additional information | no inhibition by EGTA and EDTA | Thermotoga maritima MSB8 | |
| Zn2+ | - |
Thermotoga maritima MSB8 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.26 | - |
4-nitrophenyl alpha-maltopentaoside | pH 6.5, 85°C, recombinant enzyme | Thermotoga maritima MSB8 | |
| 7.41 | - |
maltoheptaose | pH 6.5, 85°C, recombinant enzyme | Thermotoga maritima MSB8 | |
| 7.77 | - |
maltohexaose | pH 6.5, 85°C, recombinant enzyme | Thermotoga maritima MSB8 | |
| 9.95 | - |
maltopentaose | pH 6.5, 85°C, recombinant enzyme | Thermotoga maritima MSB8 | |
| 11.84 | - |
maltotetraose | pH 6.5, 85°C, recombinant enzyme | Thermotoga maritima MSB8 | |
| 14.53 | - |
beta-cyclodextrin | pH 6.5, 85°C, recombinant enzyme | Thermotoga maritima MSB8 | |
| 20.64 | - |
maltotriose | pH 6.5, 85°C, recombinant enzyme | Thermotoga maritima MSB8 | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acarbose + 2 H2O | Thermotoga maritima MSB8 | - |
acarviosine + 2 D-glucose | - |
? | |
| alpha-cyclodextrin + H2O | Thermotoga maritima MSB8 | - |
maltose + D-glucose | - |
? | |
| beta-cyclodextrin + H2O | Thermotoga maritima MSB8 | - |
maltose + D-glucose | - |
? | |
| gamma-cyclodextrin + H2O | Thermotoga maritima MSB8 | - |
maltose + D-glucose | - |
? | |
| maltoheptaose + H2O | Thermotoga maritima MSB8 | - |
maltose + D-glucose + ? | - |
? | |
| maltohexaose + H2O | Thermotoga maritima MSB8 | - |
maltose + D-glucose + ? | - |
? | |
| maltopentaose + H2O | Thermotoga maritima MSB8 | - |
maltose + D-glucose + ? | - |
? | |
| maltotetraose + H2O | Thermotoga maritima MSB8 | - |
maltose + 2 D-glucose | - |
? | |
| maltotriose + H2O | Thermotoga maritima MSB8 | - |
maltose + D-glucose | - |
? | |
| additional information | Thermotoga maritima MSB8 | TMG is an exo-acting glucosidase possessing the characteristics of both CD-/pullulan hydrolyzing enzyme and alpha-glucosidase. It hydrolyzes various maltodextrins including maltotriose to maltoheptaose and cyclomaltodextrins to mainly glucose and maltose, but it cannot hydrolyze pullulan, but acarbose | ? | - |
? | |
| soluble starch + H2O | Thermotoga maritima MSB8 | - |
maltose + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima MSB8 | Q9X2F4 | gene TM1835; gene TM1835 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant TMG 18.2fold from Escherichia coli strain MC1061 by heat treatment, hydrophobic interaction and anion exchange chromatography | Thermotoga maritima MSB8 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.24 | - |
purified recombinant enzyme, substrate soluble starch | Thermotoga maritima MSB8 |
| 4.85 | - |
purified recombinant enzyme, substrate beta-cyclodextrin | Thermotoga maritima MSB8 |
| 18.6 | - |
purified recombinant enzyme, substrate maltotriose | Thermotoga maritima MSB8 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-maltopentaoside | - |
Thermotoga maritima MSB8 | maltopentaose + 4-nitrophenol | - |
? | |
| 4-nitrophenyl alpha-maltopentaoside + H2O | - |
Thermotoga maritima MSB8 | maltopentaose + 4-nitrophenol | further hydrolysis of maltopentaose by TMG | ? | |
| acarbose + 2 H2O | - |
Thermotoga maritima MSB8 | acarviosine + 2 D-glucose | - |
? | |
| alpha-cyclodextrin + H2O | - |
Thermotoga maritima MSB8 | maltose + D-glucose | - |
? | |
| beta-cyclodextrin + H2O | - |
Thermotoga maritima MSB8 | maltose + D-glucose | - |
? | |
| gamma-cyclodextrin + H2O | - |
Thermotoga maritima MSB8 | maltose + D-glucose | - |
? | |
| malto-heptaose + H2O | - |
Thermotoga maritima MSB8 | maltose + D-glucose | - |
? | |
| malto-hexaose + H2O | - |
Thermotoga maritima MSB8 | maltose + D-glucose | - |
? | |
| malto-pentaose + H2O | - |
Thermotoga maritima MSB8 | maltose + D-glucose | - |
? | |
| malto-tetraose + H2O | - |
Thermotoga maritima MSB8 | maltose + D-glucose | - |
? | |
| malto-triose + H2O | - |
Thermotoga maritima MSB8 | maltose + D-glucose | - |
? | |
| maltoheptaose + H2O | - |
Thermotoga maritima MSB8 | maltose + D-glucose + ? | - |
? | |
| maltohexaose + H2O | - |
Thermotoga maritima MSB8 | maltose + D-glucose + ? | - |
? | |
| maltopentaose + H2O | - |
Thermotoga maritima MSB8 | maltose + D-glucose + ? | - |
? | |
| maltotetraose + H2O | - |
Thermotoga maritima MSB8 | maltose + 2 D-glucose | - |
? | |
| maltotriose + H2O | - |
Thermotoga maritima MSB8 | maltose + D-glucose | - |
? | |
| additional information | TMG is an exo-acting glucosidase possessing the characteristics of both CD-/pullulan hydrolyzing enzyme and alpha-glucosidase. It hydrolyzes various maltodextrins including maltotriose to maltoheptaose and cyclomaltodextrins to mainly glucose and maltose, but it cannot hydrolyze pullulan, but acarbose | Thermotoga maritima MSB8 | ? | - |
? | |
| additional information | the enzyme specifically cleaves a glucose unit from the reducing end of maltooligosaccharides unlike to other glucosidases. Since its enzymatic activity is negligible if alpha-methylglucoside is present in the reducing end, the type of the residue at the reducing end of the substrate is important for the TMG activity. It hydrolyzes various maltodextrins including maltotriose to maltoheptaose and cyclomaltodextrins to mainly glucose and maltose, but it cannot hydrolyze pullulan, but acarbose | Thermotoga maritima MSB8 | ? | - |
? | |
| soluble starch + H2O | - |
Thermotoga maritima MSB8 | maltose + D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cyclodextrinase | - |
Thermotoga maritima MSB8 |
| TMG | - |
Thermotoga maritima MSB8 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
assay at | Thermotoga maritima MSB8 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 95 | - |
Thermotoga maritima MSB8 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.37 | - |
beta-cyclodextrin | pH 6.5, 85°C, recombinant enzyme | Thermotoga maritima MSB8 | |
| 3.2 | - |
maltotriose | pH 6.5, 85°C, recombinant enzyme | Thermotoga maritima MSB8 | |
| 4.09 | - |
maltoheptaose | pH 6.5, 85°C, recombinant enzyme | Thermotoga maritima MSB8 | |
| 4.34 | - |
maltotetraose | pH 6.5, 85°C, recombinant enzyme | Thermotoga maritima MSB8 | |
| 4.56 | - |
maltohexaose | pH 6.5, 85°C, recombinant enzyme | Thermotoga maritima MSB8 | |
| 5.18 | - |
maltopentaose | pH 6.5, 85°C, recombinant enzyme | Thermotoga maritima MSB8 | |
| 16.28 | - |
4-nitrophenyl alpha-maltopentaoside | pH 6.5, 85°C, recombinant enzyme | Thermotoga maritima MSB8 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Thermotoga maritima MSB8 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | - |
Thermotoga maritima MSB8 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.094 | - |
beta-cyclodextrin | pH 6.5, 85°C, recombinant enzyme | Thermotoga maritima MSB8 | |
| 0.16 | - |
maltotriose | pH 6.5, 85°C, recombinant enzyme | Thermotoga maritima MSB8 | |
| 0.37 | - |
maltotetraose | pH 6.5, 85°C, recombinant enzyme | Thermotoga maritima MSB8 | |
| 0.52 | - |
maltopentaose | pH 6.5, 85°C, recombinant enzyme | Thermotoga maritima MSB8 | |
| 0.55 | - |
maltoheptaose | pH 6.5, 85°C, recombinant enzyme | Thermotoga maritima MSB8 | |
| 0.59 | - |
maltohexaose | pH 6.5, 85°C, recombinant enzyme | Thermotoga maritima MSB8 | |
| 7.2 | - |
4-nitrophenyl alpha-maltopentaoside | pH 6.5, 85°C, recombinant enzyme | Thermotoga maritima MSB8 | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
