Cloned (Comment) | Organism |
---|---|
- |
Thermococcus kodakarensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.254 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 6.5, 80°C | Thermococcus kodakarensis | |
1.79 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.5, 80°C | Thermococcus kodakarensis | |
8.37 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 6.5, 80°C | Thermococcus kodakarensis | |
34.6 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.5, 80°C | Thermococcus kodakarensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 56000, SDS-PAGE | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | Q9YGB8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Thermococcus kodakarensis | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermococcus kodakarensis | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-mannopyranoside + H2O | - |
Thermococcus kodakarensis | 4-nitrophenol + beta-D-mannopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Thermococcus kodakarensis | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
cellobiose + H2O | - |
Thermococcus kodakarensis | 2 D-glucose | - |
? | |
cellopentaose + 4 H2O | - |
Thermococcus kodakarensis | 5 D-glucose | - |
? | |
cellotetraose + 3 H2O | - |
Thermococcus kodakarensis | 4 D-glucose | - |
? | |
cellotriose + 2 H2O | - |
Thermococcus kodakarensis | 3 D-glucose | - |
? | |
additional information | exo-type beta-glycosidase. The enzyme also harbors transglycosylation activity | Thermococcus kodakarensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, SDS-PAGE | Thermococcus kodakarensis |
Synonyms | Comment | Organism |
---|---|---|
Pyrococcus kodakaraensis beta-glycosidase | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
assay at | Thermococcus kodakarensis |
100 | - |
- |
Thermococcus kodakarensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-life: more than 22 h | Thermococcus kodakarensis |
100 | - |
half-life: less than 1 h | Thermococcus kodakarensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
186 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 6.5, 80°C | Thermococcus kodakarensis | |
243 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 6.5, 80°C | Thermococcus kodakarensis | |
1650 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.5, 80°C | Thermococcus kodakarensis | |
3270 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.5, 80°C | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Thermococcus kodakarensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 6.5, 80°C | Thermococcus kodakarensis | |
94 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.5, 80°C | Thermococcus kodakarensis | |
922 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.5, 80°C | Thermococcus kodakarensis | |
957 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 6.5, 80°C | Thermococcus kodakarensis |