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Literature summary for 3.2.1.B34 extracted from

  • D'Auria, S.; Nucci, R.; Rossi, M.; Bertoli, E.; Tanfani, F.; Gryczynski, I.; Malak, H.; Lakowicz, J.R.
    beta-Glycosidase from the hyperthermophilic archaeon Sulfolobus solfataricus: structure and activity in the presence of alcohols (1999), J. Biochem., 126, 545-552.
    View publication on PubMed
Search for more literature for 3.2.1.B34

Activating Compound

Activating Compound Comment Organism Structure
1-butanol maximal activation is induced by 80 mM 1-butanol (130%). The activation at 30"C becomes less with increasing temperature and at 75°C is only 10%. Alcohol modifies the protein microenvironment, leading to a more flexible enzyme structure, which is probably responsible for the enhanced enzymatic activity Sulfolobus acidocaldarius
1-propanol alcohol modifies the protein microenvironment, leading to a more flexible enzyme structure, which is probably responsible for the enhanced enzymatic activity Sulfolobus acidocaldarius
ethanol alcohol modifies the protein microenvironment, leading to a more flexible enzyme structure, which is probably responsible for the enhanced enzymatic activity Sulfolobus acidocaldarius
methanol alcohol modifies the protein microenvironment, leading to a more flexible enzyme structure, which is probably responsible for the enhanced enzymatic activity Sulfolobus acidocaldarius

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Sulfolobus acidocaldarius

General Stability

General Stability Organism
stable in the presence of detergents Sulfolobus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 30°C, presence of 80 mM 1-butanol Sulfolobus acidocaldarius
1
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 30°C, absence of 1-butanol Sulfolobus acidocaldarius
4
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 75°C, absence of 1-butanol Sulfolobus acidocaldarius
11
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 75°C, presence of 80 mM 1-butanol Sulfolobus acidocaldarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
240000
-
-
 Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Sulfolobus acidocaldarius 2-nitrophenol + beta-D-galactopyranose
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Sulfolobus acidocaldarius

Synonyms

Synonyms Comment Organism
Sbetagly
-
 Sulfolobus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
 Sulfolobus acidocaldarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
24
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 30°C, absence of 1-butanol Sulfolobus acidocaldarius
55
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 30°C, presence of 80 mM 1-butanol Sulfolobus acidocaldarius
1320
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 75°C, absence of 1-butanol Sulfolobus acidocaldarius
1450
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 75°C, presence of 80 mM 1-butanol Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Sulfolobus acidocaldarius

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
24
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 30°C, absence of 1-butanol Sulfolobus acidocaldarius
110
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 30°C, presence of 80 mM 1-butanol Sulfolobus acidocaldarius
131.9
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 75°C, presence of 80 mM 1-butanol Sulfolobus acidocaldarius
330
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 75°C, absence of 1-butanol Sulfolobus acidocaldarius