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Literature summary for 3.2.1.B3 extracted from

  • Lunsford, R.; Macrina, F.
    Molecular cloning and characterization of scrB, the structural gene for the Streptococcus mutans phosphoenolpyruvate-dependent sucrose phosphotransferase system sucrose-6-phosphate hydrolase (1986), J. Bacteriol., 166, 426-434.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptococcus mutans serotype c

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
beta-D-fructofuranosyl 6-O-phosphono-alpha-D-glucopyranoside pH 7.1, 37°C Streptococcus mutans serotype c

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
-
Streptococcus mutans serotype c

Organism

Organism UniProt Comment Textmining
Streptococcus mutans serotype c P13522
-
-
Streptococcus mutans serotype c UA159 P13522
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-fructofuranosyl 6-O-phosphono-alpha-D-glucopyranoside + H2O
-
Streptococcus mutans serotype c alpha-D-glucose 6-phosphate + beta-D-fructose
-
?
beta-D-fructofuranosyl 6-O-phosphono-alpha-D-glucopyranoside + H2O
-
Streptococcus mutans serotype c UA159 alpha-D-glucose 6-phosphate + beta-D-fructose
-
?

Subunits

Subunits Comment Organism
? x * 57000, recombinant ScrB Streptococcus mutans serotype c
More in wild-type Streptococcus mutans, the protein is present as a 43,000-Mr species Streptococcus mutans serotype c

Synonyms

Synonyms Comment Organism
scrB sucrose-6-phosphate hydrolase component of the Streptococcus mutans phosphoenolpyruvate-dependent sucrose phosphotransferase system Streptococcus mutans serotype c

General Information

General Information Comment Organism
physiological function scrB-defective strains exhibit sucrose sensitivity Streptococcus mutans serotype c