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Literature summary for 3.2.1.B3 extracted from

  • Engels, V.; Georgi, T.; Wendisch, V.F.
    ScrB (Cg2927) is a sucrose-6-phosphate hydrolase essential for sucrose utilization by Corynebacterium glutamicum (2008), FEMS Microbiol. Lett., 289, 80-89.
    View publication on PubMed
Search for more literature for 3.2.1.B3

Activating Compound

Activating Compound Comment Organism Structure
fructose 6-phosphate 1.5 fold stimulation, 10 mM Corynebacterium glutamicum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha and BL21(DE3), plasmids pGEM-T, pVWEx1, pET16b, recombinant protein and deletion mutant Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ CaCl2 inhibits activity of, 5 mM, 10% inhibition Corynebacterium glutamicum
fructose reduced to 70% by the presence of 100 mM Corynebacterium glutamicum
Mn2+ MnCl2 inhibits activity of, 5 mM, 26% inhibition Corynebacterium glutamicum
Zn2+ ZnSO4 inhibits activity of, 5 mM, 98% inhibition Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 sucrose 6-phosphate recombinant protein, pH 6.5, at 30°C Corynebacterium glutamicum
190
-
 sucrose recombinant protein, pH 6.5, at 30°C Corynebacterium glutamicum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 20% stimulation by MgSO4, 5 mM Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum Q8NMD5 encoded by cg2927 gene that corresponds to the scrB gene; ATCC13032 as wild-type strain, deletion mutant
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 cg2927 gene corresponds to the scrB gene and encodes sucrose 6-phosphate hydrolase, recombinant protein hydrolyzes sucrose 6-phosphate, sucrose 6-phosphate much more effective than sucrose as substrate, deletion mutant does not grow on sucrose as the sole carbon source, growth in the absence of enzyme activity severely decreased when sucrose is present in addition to glucose, fructose or acetate, transcriptional start sites analyzed, sucrose 6-phosphate hydrolase activity increased during growth in fructose- or sucrose-containing media, overexpression increases hydrolase activity sevenfold, no activity in the scrB deletion mutant Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + H2O
-
 Corynebacterium glutamicum D-glucose + D-fructose
-
 ?
sucrose 6-phosphate + H2O no activity towards sucrose 6'-phosphate Corynebacterium glutamicum D-glucose 6-phosphate + D-fructose
-
 ?

Synonyms

Synonyms Comment Organism
scrB protein
-
 Corynebacterium glutamicum
sucrose 6-phosphate hydrolase
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 temperature optimum of ScrB protein Corynebacterium glutamicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at, optimum between 6.5 and 7.0 at 30°C Corynebacterium glutamicum

pH Range

pH Minimum pH Maximum Comment Organism
5 8 13% activity at pH 5.0, 45% activity at pH 8.5 if compared to activity at pH 6.5, no activity below pH 3.0 Corynebacterium glutamicum