Literature summary for 3.2.1.B3 extracted from

Thompson, J.; Robrish, S.A.; Immel, S.; Lichtenthaler, F.W.; Hall, B.G.; Pikis, A.
Metabolism of sucrose and its five linkage-isomeric alpha-D-glucosyl-D-fructoses by Klebsiella pneumoniae. Participation and properties of sucrose-6-phosphate hydrolase and phospho-alpha-glucosidase (2001), J. Biol. Chem., 276, 37415-37425.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Klebsiella pneumoniae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0853	-	beta-D-fructofuranosyl 6-O-phosphono-alpha-D-glucopyranoside	pH 7.5	Klebsiella pneumoniae
20.3	-	sucrose	pH 7.5	Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
53000	-	SDS-PAGE	Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sucrose 6-phosphate + H2O	Klebsiella pneumoniae	metabolism of sucrose, alpha-D-glucose 6-phosphate + beta-D-fructose are metabolized via the glycolytic pathway to yíeld primary lactic acid	alpha-D-glucose 6-phosphate + beta-D-fructose	-	?

Organism

Organism	UniProt	Comment	Textmining
Klebsiella pneumoniae	P27217	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-D-fructofuranosyl 6-O-phosphono-alpha-D-glucopyranoside + H2O + H2O	i.e. sucrose 6-phosphate	Klebsiella pneumoniae	alpha-D-glucose 6-phosphate + beta-D-fructose	-	?
additional information	no detectable hydrolysis (at 1 mM) of any of the phosphorylated isomers of sucrose, the enzyme fails to hydrolyze other phospho-alpha-glucosides including maltose 6'-phosphate and trehalose 6-phosphate	Klebsiella pneumoniae	?	-	?
sucrose + H2O	-	Klebsiella pneumoniae	alpha-D-glucose + beta-D-fructose	-	?
sucrose 6-phosphate + H2O	metabolism of sucrose, alpha-D-glucose 6-phosphate + beta-D-fructose are metabolized via the glycolytic pathway to yíeld primary lactic acid	Klebsiella pneumoniae	alpha-D-glucose 6-phosphate + beta-D-fructose	-	?

Subunits

Subunits	Comment	Organism
?	x * 53000, SDS-PAGE	Klebsiella pneumoniae

Synonyms

Synonyms	Comment	Organism
scrB	-	Klebsiella pneumoniae
sucrose-6-P hydrolase	-	Klebsiella pneumoniae
sucrose-6-P-hydrolase	-	Klebsiella pneumoniae
Sucrose-6-phosphate hydrolase	-	Klebsiella pneumoniae

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Release 2023.1 (January 2023)