Application | Comment | Organism |
---|---|---|
additional information | the enzyme converts flavanone glycoside to flavanone aglycone via a one-step reaction. It may be useful in the production of the flavanone aglycones naringenin and hesperetin from flavanone glycosides in citrus extracts | Pyrococcus furiosus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-methyl-1-butanol | 10% (v/v), about% inhibition | Pyrococcus furiosus | |
2-propanol | 10% (v/v), about% inhibition | Pyrococcus furiosus | |
acetone | 10% (v/v), about% inhibition | Pyrococcus furiosus | |
DMSO | 10% (v/v), about% inhibition | Pyrococcus furiosus | |
ethanol | 10% (v/v), about 50% inhibition | Pyrococcus furiosus | |
formate | 10% (v/v), about% inhibition | Pyrococcus furiosus | |
Isopropanol | 10% (v/v), about% inhibition | Pyrococcus furiosus | |
methanol | 10% (v/v), about% inhibition | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
hesperidin | pH 5.5, 95°C | Pyrococcus furiosus | |
1.7 | - |
naringin | pH 5.5, 95°C | Pyrococcus furiosus | |
3.7 | - |
narirutin | pH 5.5, 95°C | Pyrococcus furiosus | |
4.6 | - |
neohesperidin | pH 5.5, 95°C | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55488 | - |
x * 55488, calculated from sequence | Pyrococcus furiosus |
56000 | - |
x * 56000, SDS-PAGE | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q51723 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.011 | - |
substrate: rutin, pH 5.5, 95°C | Pyrococcus furiosus |
0.081 | - |
substrate: neoponcirin, pH 5.5, 95°C | Pyrococcus furiosus |
0.085 | - |
substrate: diosmin, pH 5.5, 95°C | Pyrococcus furiosus |
0.091 | - |
substrate: poncirin, pH 5.5, 95°C | Pyrococcus furiosus |
0.102 | - |
substrate naringin, pH 5.5, 95°C | Pyrococcus furiosus |
0.103 | - |
substrate: neohesperidin, pH 5.5, 95°C | Pyrococcus furiosus |
0.153 | - |
substrate: narirutin, pH 5.5, 95°C | Pyrococcus furiosus |
0.172 | - |
substrate: hesperidin, pH 5.5, 95°C | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diosmin + H2O | - |
Pyrococcus furiosus | diosmetin + rutinose | - |
? | |
hesperidin + H2O | - |
Pyrococcus furiosus | hesperetin + rutinose | - |
? | |
naringin + H2O | - |
Pyrococcus furiosus | naringenin + rutinose | - |
? | |
narirutin + H2O | - |
Pyrococcus furiosus | naringenin + neohesperidose | - |
? | |
neohesperidin + H2O | - |
Pyrococcus furiosus | hesperetin + neohesperidose | - |
? | |
neoponcirin + H2O | - |
Pyrococcus furiosus | isosakuranetin + rutinose | - |
? | |
poncirin + H2O | - |
Pyrococcus furiosus | isosakuranetin + neohesperidose | - |
? | |
rutin + H2O | - |
Pyrococcus furiosus | quercetin + rutinose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, SDS-PAGE | Pyrococcus furiosus |
? | x * 55488, calculated from sequence | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | 95 | the activity increases from 75°C to 95°C, activity at 75°C is about 40% of the activity at 95°C, substrate: hesperidin | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
half-life: 637 h | Pyrococcus furiosus |
80 | - |
half-life: 392 h | Pyrococcus furiosus |
85 | - |
half-life: 235 h | Pyrococcus furiosus |
90 | - |
half-life: 138 h | Pyrococcus furiosus |
95 | - |
half-life: 88 h | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
27.7 | - |
naringin | pH 5.5, 95°C | Pyrococcus furiosus | |
68.4 | - |
hesperidin | pH 5.5, 95°C | Pyrococcus furiosus | |
74.1 | - |
narirutin | pH 5.5, 95°C | Pyrococcus furiosus | |
84.8 | - |
neohesperidin | pH 5.5, 95°C | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
substrate: hesperidin | Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6.5 | pH 4.5: about 50% of maximal activity, pH 6.5: about 60% of maximal activity, substrate: hesperidin | Pyrococcus furiosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.5 | - |
naringin | pH 5.5, 95°C | Pyrococcus furiosus | |
18.5 | - |
neohesperidin | pH 5.5, 95°C | Pyrococcus furiosus | |
19.8 | - |
narirutin | pH 5.5, 95°C | Pyrococcus furiosus | |
42.1 | - |
hesperidin | pH 5.5, 95°C | Pyrococcus furiosus |