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Literature summary for 3.2.1.B26 extracted from

  • Huneke, F.-U.; Nucci, R.; Cowan, D.
    Effect of water miscible organic solvents on kinetics of a thermostable beta-glycosidase (1999), Biocatal. Biotransform., 17, 251-267.
No PubMed abstract available
Search for more literature for 3.2.1.B26

Activating Compound

Activating Compound Comment Organism Structure
acetonitrile maximal increase in enzyme activity at solvent concentrations of 15% (v/v) with 4-nitrophenyl beta-D-fucopyranoside as substrate Saccharolobus solfataricus
butan-2-one maximum increase in enzyme activity (1.5fold compared to activity in buffer) is measured at 5% (v/v) solvent concentrations with 4-nitrophenyl beta-D-fucopyranoside as substrate Saccharolobus solfataricus
methyl acetate 5% (v/v), increases activity with 2-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-fucopyranoside or 4-nitrophenyl beta-D-glucopyranoside Saccharolobus solfataricus
methyl acetate highest activation at solvent concentrations of 5% (v/v) with 4-nitrophenyl beta-D-fucopyranoside as substrate Saccharolobus solfataricus
tetrahydrofuran 5% (v/v), increases activity with 2-nitrophenyl beta-D-galactopyranoside or 4-nitrophenyl beta-D-glucopyranoside Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
1,4-dioxane 10% (v/v), decreases activity with 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-glucopyranoside or 4-nitrophenyl beta-D-fucopyranoside Saccharolobus solfataricus
butan-2-one 10-20 (v/v) Saccharolobus solfataricus
D-gluconic acid lactone strong competitive inhibitor Saccharolobus solfataricus
tetrahydrofuran 5% (v/v), decreases activity with 4-nitrophenyl beta-D-galactopyranoside or 4-nitrophenyl beta-D-fucopyranoside Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.095
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.5, 60°C Saccharolobus solfataricus
0.2
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.5, 60°C Saccharolobus solfataricus
0.83
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.5, 60°C Saccharolobus solfataricus
1.14
-
 2-nitrophenyl beta-D-galactopyranoside pH 6.5, 60°C Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus 2-nitrophenol + beta-D-galactopyranose
-
 ?
4-nitrophenyl beta-D-fucopyranoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + beta-D-fucopyranose
-
 ?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + beta-D-galactopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + D-glucopyranose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 72 h, stable Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
462
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.5, 60°C Saccharolobus solfataricus
940
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.5, 60°C Saccharolobus solfataricus
2374
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.5, 60°C Saccharolobus solfataricus
2437
-
 2-nitrophenyl beta-D-galactopyranoside pH 6.5, 60°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.025
-
 D-gluconic acid lactone pH 6.5, 60°C Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2137
-
 2-nitrophenyl beta-D-galactopyranoside pH 6.5, 60°C Saccharolobus solfataricus
2860
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.5, 60°C Saccharolobus solfataricus
4700
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.5, 60°C Saccharolobus solfataricus
4863
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.5, 60°C Saccharolobus solfataricus