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Literature summary for 3.2.1.99 extracted from
- GH43 endo-arabinanase from Bacillus licheniformis Structure, activity and unexpected synergistic effect on cellulose enzymatic hydrolysis (2018), Int. J. Biol. Macromol., 117, 7-16 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus licheniformis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Bacillus licheniformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Bacillus licheniformis |  |
| EDTA | - |
Bacillus licheniformis | |
| Ni2+ | - |
Bacillus licheniformis | |
| Zn2+ | - |
Bacillus licheniformis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activity is partially dependent of a bound Ca2+ since 70% of the maximal activity is preserved even when 1 mM EDTA is added to the reaction medium | Bacillus licheniformis | |
| Co2+ | increases activity | Bacillus licheniformis | |
| Mg2+ | increases activity | Bacillus licheniformis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46769 | - |
calculated from sequence | Bacillus licheniformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | Q65GB9 | - |
- |
| Bacillus licheniformis ATCC 14580 | Q65GB9 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| linear arabinan + H2O | the enzyme is selective for linear arabinan | Bacillus licheniformis | ? | - |
? | |
| linear arabinan + H2O | the enzyme is selective for linear arabinan | Bacillus licheniformis ATCC 14580 | ? | - |
? | |
| additional information | the enzyme shows a synergic effect with the commercial cocktail Accellerase 1500 on cellulose hydrolysis. It weakens the interaction between cellulose fibers in filter paper, thus providing an increased access to the cellulases of the cocktail | Bacillus licheniformis | ? | - |
? | |
| additional information | the enzyme shows a synergic effect with the commercial cocktail Accellerase 1500 on cellulose hydrolysis. It weakens the interaction between cellulose fibers in filter paper, thus providing an increased access to the cellulases of the cocktail | Bacillus licheniformis ATCC 14580 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BlAbn1 | - |
Bacillus licheniformis |
| GH43 endo-arabinanase | - |
Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Bacillus licheniformis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | 30°C: about 35% of maximal activity, 60°C: about 40% of maximal activity | Bacillus licheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
adding CoCl2 hexahydrate results in a marked increase in melting temperature, of almost 10°C. Strontium chloride also stabilizes BlAbn1 against thermal denaturation | Bacillus licheniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacillus licheniformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | pH 7.0: about 55% of maximal activity, pH 9.0: about 20% of maximal activity | Bacillus licheniformis |
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Release 2023.1 (January 2023)
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