Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus licheniformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, recombinant enzyme, SDS-PAGE | Bacillus licheniformis |
36358 | - |
x * 36358, calculated from amino acid sequence | Bacillus licheniformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-alpha-L-arabinan + H2O | Bacillus licheniformis | - |
oligo-alpha-(1->5)-L-arabinoside | - |
? | |
1,5-alpha-L-arabinan + H2O | Bacillus licheniformis DSM 13 | - |
oligo-alpha-(1->5)-L-arabinoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | Q65GB9 | - |
- |
Bacillus licheniformis | Q65L63 | - |
- |
Bacillus licheniformis DSM 13 | Q65GB9 | - |
- |
Bacillus licheniformis DSM 13 | Q65L63 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Bacillus licheniformis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.13 | - |
sugar beet arabinan as substrate, in 50 mM sodium acetate buffer (pH 6.0) at 55°C | Bacillus licheniformis |
64.52 | - |
with linear 1,5-alpha-L-arabinan as substrate, in 50 mM sodium acetate buffer (pH 6.0) at 55°C | Bacillus licheniformis |
71.69 | - |
with debranched 1,5-alpha-L-arabinan as substrate, in 50 mM sodium acetate buffer (pH 6.0) at 55°C | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-alpha-L-arabinan + H2O | - |
Bacillus licheniformis | oligo-alpha-(1->5)-L-arabinoside | - |
? | |
1,5-alpha-L-arabinan + H2O | - |
Bacillus licheniformis DSM 13 | oligo-alpha-(1->5)-L-arabinoside | - |
? | |
debranched 1,5-alpha-L-arabinan + H2O | highest activity | Bacillus licheniformis | ? | - |
? | |
debranched 1,5-alpha-L-arabinan + H2O | highest activity | Bacillus licheniformis DSM 13 | ? | - |
? | |
linear 1,5-alpha-L-arabinan + H2O | - |
Bacillus licheniformis | ? | - |
? | |
linear 1,5-alpha-L-arabinan + H2O | - |
Bacillus licheniformis DSM 13 | ? | - |
? | |
additional information | the enzyme randomly cleaves the internal alpha-(1,5)-linked L-arabinofuranosyl residues of a branchless arabinan backbone to release arabinotriose mainly, although a small amount of arabino-oligosaccharide intermediates is also liberated | Bacillus licheniformis | ? | - |
? | |
additional information | the enzyme randomly cleaves the internal alpha-(1,5)-linked L-arabinofuranosyl residues of a branchless arabinan backbone to release arabinotriose mainly, although a small amount of arabino-oligosaccharide intermediates is also liberated | Bacillus licheniformis DSM 13 | ? | - |
? | |
sugar beet arabinan + H2O | weak activity | Bacillus licheniformis | ? | - |
? | |
sugar beet arabinan + H2O | weak activity | Bacillus licheniformis DSM 13 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, recombinant enzyme, SDS-PAGE | Bacillus licheniformis |
? | x * 36358, calculated from amino acid sequence | Bacillus licheniformis |
Synonyms | Comment | Organism |
---|---|---|
abnA1 | isoform | Bacillus licheniformis |
abnA2 | isoform | Bacillus licheniformis |
ABNase | - |
Bacillus licheniformis |
endo-(1,5)-alpha-L-arabinanase | - |
Bacillus licheniformis |
endo-arabinanase | - |
Bacillus licheniformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Bacillus licheniformis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | about 45% activity at 30°C, about 65% activity at 40°C, about 85% activity at 50°C, about 80% activity at 60°C, and about 20% activity at 70°C | Bacillus licheniformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | the half-life of the enzyme at 50, 55, and 60°C are 280, 152, and 30 min, respectively | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Bacillus licheniformis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 6.5 | activity is markedly reduced to about 50% at pH 5.0 and at pH 6.5, respectively | Bacillus licheniformis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 12 | the enzyme shows more than 60% activity at pH 5.0-11.0 after 1 h incubation | Bacillus licheniformis |