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Literature summary for 3.2.1.99 extracted from
- Characterization of a thermostable endo-1,5-alpha-L-arabinanase from Caldicellulorsiruptor saccharolyticus (2009), Biotechnol. Lett., 31, 1439-1443.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Caldicellulosiruptor saccharolyticus DSM 8903 |  |
| Co2+ | - |
Caldicellulosiruptor saccharolyticus DSM 8903 | |
| Fe2+ | - |
Caldicellulosiruptor saccharolyticus DSM 8903 | |
| Mg2+ | - |
Caldicellulosiruptor saccharolyticus DSM 8903 | |
| Mn2+ | - |
Caldicellulosiruptor saccharolyticus DSM 8903 | |
| Ni2+ | - |
Caldicellulosiruptor saccharolyticus DSM 8903 | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli | Caldicellulosiruptor saccharolyticus DSM 8903 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Caldicellulosiruptor saccharolyticus DSM 8903 | |
| Cu2+ | - |
Caldicellulosiruptor saccharolyticus DSM 8903 | |
| Zn2+ | - |
Caldicellulosiruptor saccharolyticus DSM 8903 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
2 * 56000, recombinant His-tagged enzyme, SDS-PAGE, 2 x 56268, sequence calculation | Caldicellulosiruptor saccharolyticus DSM 8903 |
| 112000 | - |
recombinant His-tagged enzyme, gel filtration | Caldicellulosiruptor saccharolyticus DSM 8903 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldicellulosiruptor saccharolyticus DSM 8903 | A4XJR7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme 39fold from Escherichia coli by nickel affinity chromatography | Caldicellulosiruptor saccharolyticus DSM 8903 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.6 | - |
purified recombinant enzyme, substrate is sugar beet arabinan | Caldicellulosiruptor saccharolyticus DSM 8903 |
| 12 | - |
purified recombinant enzyme, substrate 1,5-alpha-L-arabinan | Caldicellulosiruptor saccharolyticus DSM 8903 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,5-alpha-L-arabinan + H2O | debranched arabinan, best substrate | Caldicellulosiruptor saccharolyticus DSM 8903 | ? | - |
? | |
| additional information | no activity for arabinoxylan, 1,4-beta-D-xylose units with side chains of 1,3-alpha-L-arabinose, xylan, starch, or 4-nitrophenyl-alpha-L-arabinofuranoside, a typical substrate for the arabinofuranosidase. Synergistic hydroysis of arabinan by endo-1,5-alpha-L-arabinanase and alpha-L-arabinofuranosidase | Caldicellulosiruptor saccharolyticus DSM 8903 | ? | - |
? | |
| sugar beet arabinan + H2O | the substrate is composed of 1,2-alpha- and 1,3-alpha-L-arabinofuranosyl side chains linked to a 1,5-alpha-L-arabinofuranosyl backbone | Caldicellulosiruptor saccharolyticus DSM 8903 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 56000, recombinant His-tagged enzyme, SDS-PAGE, 2 x 56268, sequence calculation | Caldicellulosiruptor saccharolyticus DSM 8903 |
| More | structure modelling and analysis, overview | Caldicellulosiruptor saccharolyticus DSM 8903 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-1,5-alpha-L-arabinanase | - |
Caldicellulosiruptor saccharolyticus DSM 8903 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Caldicellulosiruptor saccharolyticus DSM 8903 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 85 | - |
Caldicellulosiruptor saccharolyticus DSM 8903 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
purified recombinant His-tagged enzyme, half-life is 2440 h | Caldicellulosiruptor saccharolyticus DSM 8903 |
| 70 | - |
purified recombinant His-tagged enzyme, half-life is 254 h | Caldicellulosiruptor saccharolyticus DSM 8903 |
| 75 | - |
purified recombinant His-tagged enzyme, half-life is 93 h | Caldicellulosiruptor saccharolyticus DSM 8903 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 50 | - |
1,5-alpha-L-arabinan | recombinant enzyme, pH 6.5, 75°C | Caldicellulosiruptor saccharolyticus DSM 8903 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Caldicellulosiruptor saccharolyticus DSM 8903 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | - |
Caldicellulosiruptor saccharolyticus DSM 8903 |
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