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Literature summary for 3.2.1.99 extracted from

  • Squina, F.M.; Prade, R.A.; Wang, H.; Murakami, M.T.
    Expression, purification, crystallization and preliminary crystallographic analysis of an endo-1,5-alpha-L-arabinanase from hyperthermophilic Thermotoga petrophila (2009), Acta Crystallogr. Sect. F, 65, 902-905.
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
industry endo-1,5-alpha-L-arabinanases belonging to glycoside hydrolase family 43 are of great industrial interest for use in food technology, organic synthesis and biofuel production owing to their ability to catalyze the hydrolysis of alpha-1,5-arabinofuranosidic bonds in arabinose-containing polysaccharides Thermotoga petrophila

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of His-tagged enzyme in Escherichia coli Thermotoga petrophila

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged enzyme, 0.0005 ml of protein solution containing 30 mg/ml in 25 mM Tris-HCl buffer, pH 7, 5, is mixed with 0.0005 ml of reservoir solution containing 0.1 M MES buffer, pH 6.5, 0.8 M ammonium sulfate, 0.1 M EDTA, 0.1 M L-proline and 5% v/v dioxane, X-ray diffraction structure determination and analysis at 2.86 A resolution Thermotoga petrophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Thermotoga petrophila the enzyme shows the ability to catalyze the hydrolysis of alpha-1,5-arabinofuranosidic bonds in arabinose-containing polysaccharides ?
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 ?

Organism

Organism UniProt Comment Textmining
Thermotoga petrophila
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli Thermotoga petrophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows the ability to catalyze the hydrolysis of alpha-1,5-arabinofuranosidic bonds in arabinose-containing polysaccharides Thermotoga petrophila ?
-
 ?

Synonyms

Synonyms Comment Organism
endo-1,5-alpha-L-arabinanase
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 Thermotoga petrophila
More the enzyme belongs to the glycoside hydrolase family 43, GH43 Thermotoga petrophila