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Literature summary for 3.2.1.99 extracted from

  • Leal, T.L.; de Sa-Nogueira I.
    Purification, characterization and functional analysis of an endo-arabinanase (AbnA) from Bacillus subtilis (2004), FEMS Microbiol. Lett., 241, 41-48.
    View publication on PubMed
Search for more literature for 3.2.1.99

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.92
-
 linear 1,5-alpha-L-arabinan
-
 Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Bacillus subtilis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose affinity chromatography Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 no detectable activity against larch wood arabinoxylan, wheat arabinoxylan or p-nitrophenyl-alpha-L-arabinofuranoside Bacillus subtilis
116
-
 sugar beet arabinan, at 37°C, pH 6.6 Bacillus subtilis
366
-
 linear 1,5-alpha-L-arabinan, at 37°C, pH 6.6 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linear 1,5-alpha-L-arabinan + H2O
-
 Bacillus subtilis L-arabinose + ?
-
 ?
sugar beet arabinan + H2O
-
 Bacillus subtilis L-arabinose + ?
-
 ?

Synonyms

Synonyms Comment Organism
ABN
-
 Bacillus subtilis
ABNA
-
 Bacillus subtilis
endo-1,5-alpha-L-arabinanase
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 at pH 6.6 Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 at 37°C Bacillus subtilis