Any feedback?
Literature summary for 3.2.1.99 extracted from
- Purification and characterization of thermostable endo-1,5-alpha-L-arabinase from a strain of Bacillus thermodenitrificans (2002), Appl. Environ. Microbiol., 68, 1639-1646.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag2+ | complete inhibition at 1 mM | Geobacillus thermodenitrificans |  |
| Hg2+ | complete inhibition at 1 mM | Geobacillus thermodenitrificans | |
| additional information | no inhibition by Ba2+, Mn2+ and Mg2+ | Geobacillus thermodenitrificans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Geobacillus thermodenitrificans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
SDS-PAGE | Geobacillus thermodenitrificans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus thermodenitrificans | AB063312 | - |
- |
| Geobacillus thermodenitrificans TS-3 | AB063312 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 7400fold | Geobacillus thermodenitrificans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 445 | - |
- |
Geobacillus thermodenitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,5-alpha-L-arabinan + H2O | i.e. debranched arabinan | Geobacillus thermodenitrificans | alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara | - |
? | |
| 1,5-alpha-L-arabinan + H2O | i.e. debranched arabinan | Geobacillus thermodenitrificans TS-3 | alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara | - |
? | |
| arabinan + H2O | - |
Geobacillus thermodenitrificans | ? | - |
? | |
| arabinan + H2O | - |
Geobacillus thermodenitrificans TS-3 | ? | - |
? | |
| additional information | no substrates: carboxymethyl cellulose, xylan, polygalacturonate, pectin, galactan, 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside,4-nitrophenyl-alpha-D-xylopyranoside and 4-nitrophenyl-beta-D-cellobioside | Geobacillus thermodenitrificans | ? | - |
? | |
| additional information | no substrates: carboxymethyl cellulose, xylan, polygalacturonate, pectin, galactan, 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside,4-nitrophenyl-alpha-D-xylopyranoside and 4-nitrophenyl-beta-D-cellobioside | Geobacillus thermodenitrificans TS-3 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ABN | - |
Geobacillus thermodenitrificans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Geobacillus thermodenitrificans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
half-life: 4 h | Geobacillus thermodenitrificans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Geobacillus thermodenitrificans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | quite stable at 37°C for 16 h | Geobacillus thermodenitrificans |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Geobacillus thermodenitrificans | isoelectric focusing, pH gradient 3-10 | - |
4.5 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
