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Literature summary for 3.2.1.99 extracted from

  • Ramon, D.; van der Veen, P.; Visser, J.
    Arabinan degrading enzymes from Aspergillus nidulans: induction and purification (1993), FEMS Microbiol. Lett., 113, 15-22.
    View publication on PubMed
Search for more literature for 3.2.1.99

Activating Compound

Activating Compound Comment Organism Structure
apple pulp induction of endo-arabinase and activation Aspergillus nidulans
L-arabitol induction of endo-arabinase, addition of D-glucose prevents this induction Aspergillus nidulans
L-arabitol induction of endo-arabinase, addition of D-glucose prevents this induction Aspergillus niger
sugar beet pulp induction of endo-arabinase and activation Aspergillus nidulans
sugar beet pulp induction of endo-arabinase and activation Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic data Aspergillus nidulans
additional information
-
 additional information kinetic data Aspergillus niger
0.052
-
 1,5-alpha-L-arabinan CM-linear 1,5-alpha-L-arabinan Aspergillus niger
0.336
-
 1,5-alpha-L-arabinan CM-linear 1,5-alpha-L-arabinan Aspergillus nidulans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aspergillus nidulans
-
-
extracellular
-
 Aspergillus niger
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 SDS-PAGE Aspergillus nidulans
43000
-
 SDS-PAGE Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
 wild-type WG096
-
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus nidulans

Reaction

Reaction Comment Organism Reaction ID
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O = alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose regulation, induction and carbon catabolite repression of endo-1,5-alpha-arabinanase Aspergillus nidulans
a
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O = alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose regulation, induction and carbon catabolite repression of endo-1,5-alpha-arabinanase Aspergillus niger
a

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Aspergillus nidulans
-
culture medium
-
 Aspergillus niger
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-alpha-L-arabinan + H2O
-
 Aspergillus niger alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
 ?
1,5-alpha-L-arabinan + H2O
-
 Aspergillus nidulans L-arabinan oligomers
-
 ?
arabinogalactan + H2O
-
 Aspergillus niger ?
-
 ?
arabinogalactan + H2O very poor substrate Aspergillus nidulans ?
-
 ?
L-arabinan + H2O apple and sugar beet pulp Aspergillus nidulans L-arabino-oligosaccharides
-
 ?
L-arabinan + H2O sugar beet pulp Aspergillus nidulans L-arabino-oligosaccharides
-
 ?
L-arabinan + H2O sugar beet pulp Aspergillus niger L-arabino-oligosaccharides
-
 ?
additional information
-
 Aspergillus niger ?
-
 ?
additional information inactive on p-nitrophenyl-alpha-L-arabinofuranoside Aspergillus nidulans ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
51
-
-
 Aspergillus niger
68
-
-
 Aspergillus nidulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6
-
-
 Aspergillus niger
5.5
-
-
 Aspergillus nidulans