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Literature summary for 3.2.1.99 extracted from
- Purification and some properties of a protopectin-solubilizing enzyme that has potent activity on sugar beet protopectin (1990), Agric. Biol. Chem., 54, 879-889.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | useful for studies on structure of protopectin | Bacillus subtilis |
| nutrition | useful for pectin production from sugar beet pulp | Bacillus subtilis |
General Stability
| General Stability | Organism |
|---|---|
| IFO 3134, EDTA stabilizes during purification | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | IFO 3134, slight inhibition | Bacillus subtilis |  |
| AgNO3 | IFO 3134 | Bacillus subtilis | |
| CdCl2 | IFO 3134 | Bacillus subtilis | |
| Fe2(SO4)3 | IFO 3134 | Bacillus subtilis | |
| Hg2+ | IFO 3134, completely | Bacillus subtilis | |
| HgCl2 | IFO 3134, completely | Bacillus subtilis | |
| Mn2+ | IFO 3134, completely | Bacillus subtilis | |
| MnCl2 | IFO 3134, completely | Bacillus subtilis | |
| additional information | - |
Bacillus subtilis | |
| NaN3 | IFO 3134, slight inhibition | Bacillus subtilis | |
| NaNO2 | IFO 3134, strong inhibition | Bacillus subtilis | |
| o-phenanthroline | IFO 3134, slight inhibition | Bacillus subtilis | |
| p-chloromercuribenzoate | IFO 3134, slight inhibition | Bacillus subtilis | |
| PbCl2 | IFO 3134 | Bacillus subtilis | |
| Sn2+ | IFO 3134, strong inhibition | Bacillus subtilis | |
| SnCl2 | IFO 3134, strong inhibition | Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| BaCl2 | IFO 3134, slight activation | Bacillus subtilis | |
| CaCl2 | IFO 3134, slight activation | Bacillus subtilis | |
| CoCl2 | IFO 3134, very slight activation | Bacillus subtilis | |
| Hg2Cl2 | IFO 3134, slight activation | Bacillus subtilis | |
| MgCl2 | IFO 3134, activation | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
3134, gel filtration on Toyopearl HW-55S | Bacillus subtilis |
| 30000 | - |
IFO 3134, SDS-PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
IFO 3134 | - |
| Bacillus subtilis | - |
IFO 3022 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| IFO 3134 | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.8 | - |
IFO 3134 | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,5-alpha-L-arabinan + H2O | - |
Bacillus subtilis | alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose | - |
? | |
| cell wall polysaccharide + H2O | - |
Bacillus subtilis | L-arabinan | - |
? | |
| L-arabinan + H2O | sugar beet pulp | Bacillus subtilis | L-arabino-oligosaccharides | - |
? | |
| additional information | IFO 3134, inactive on polygalacturonic acid and soluble pectin | Bacillus subtilis | ? | - |
? | |
| protopectin + H2O | IFO 3134, protopectin of sugar beet pulp and various citrus fruit peels, but much less on that from lemon peel | Bacillus subtilis | pectin | IFO 3134, highly polymerized pectin | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | IFO 3134, 1 * 30000, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PPase-C | IFO 3134 | Bacillus subtilis |
| protopectinase C | IFO 3134 | Bacillus subtilis |
| protopectinase-C | IFO 3134 | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
IFO 3134 | Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | IFO 3134, activity between | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
IFO 3134, up to, retains more than 80% of activity after 10 min | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
IFO 3134, at 37°C | Bacillus subtilis |
| 8 | - |
IFO 3134, at 60°C | Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | IFO 3134 | Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | IFO 3134, stable between at 50°C: 30 min | Bacillus subtilis |
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