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Literature summary for 3.2.1.99 extracted from

  • McKie, V.A.; Black, G.W.; Millward-Sadler, S.J.; Hazlewood, G.P.; Laurie, J.I.; Gilbert, H.J.
    Arabinanase A from Pseudomonas fluorescens subsp. cellulosa exhibits both an endo- and an exo-mode of action (1997), Biochem. J., 323, 547-555.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas fluorescens
hyperexpression in Escherichia coli Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Pseudomonas fluorescens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Pseudomonas fluorescens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
SDS-PAGE Pseudomonas fluorescens
39440
-
calculated from nucleotide sequence Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
arabinanase A, exhibits endo- and exo-mode of action
-
Pseudomonas fluorescens
-
subspecies cellulosa
-

Purification (Commentary)

Purification (Comment) Organism
recombinant arabinanase Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-alpha-L-arabinan + H2O linear arabinan Pseudomonas fluorescens alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + ? main product ?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O poor substrate Pseudomonas fluorescens alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
Pseudomonas fluorescens alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranose
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
Pseudomonas fluorescens alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
Pseudomonas fluorescens alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
Pseudomonas fluorescens alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
Pseudomonas fluorescens alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
?
carboxymethylarabinan + H2O
-
Pseudomonas fluorescens ?
-
?
L-arabinan + H2O linear arabinan Pseudomonas fluorescens L-arabino-oligosaccharides main product: L-arabinotriose ?
L-arabinan + H2O branched sugar beet arabinan containing alpha-1,3-arabinose side chains: not a substrate Pseudomonas fluorescens L-arabino-oligosaccharides main product: L-arabinotriose ?
L-arabino-oligosaccharide + H2O
-
Pseudomonas fluorescens L-arabino-oligosaccharide main product: L-arabinotriose ?
additional information inactive on cellulose, avicel, amorphous cellulose, carboxymethylcellulose, xylan, galactan, mannan, 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl alpha-L-arabinopyranoside, 4-nitrophenyl beta-xyloside and 2,4-dinitrophenyl beta-xylobioside Pseudomonas fluorescens ?
-
?

Synonyms

Synonyms Comment Organism
arabinanase A exhibits endo- and exo-mode of action Pseudomonas fluorescens
arbA exhibits endo- and exo-mode of action Pseudomonas fluorescens