General Stability | Organism |
---|---|
Ca2+ stabilizes, enzyme is more thermostable in the presence of Ca2+ | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | extracellular enzyme, strong inhibition | Klebsiella pneumoniae | |
AgNO3 | extracellular enzyme, strong inhibition | Klebsiella pneumoniae | |
Cu2+ | extracellular enzyme, strong inhibition | Klebsiella pneumoniae | |
CuSO4 | extracellular enzyme, strong inhibition | Klebsiella pneumoniae | |
FeSO4 | extracellular enzyme | Klebsiella pneumoniae | |
Hg2+ | extracellular enzyme, completely | Klebsiella pneumoniae | |
HgCl2 | extracellular enzyme, completely | Klebsiella pneumoniae | |
iodoacetamide | extracellular enzyme, 1 mM: 11% inactivation; slight inactivation | Klebsiella pneumoniae | |
p-chloromercuribenzoate | extracellular enzyme, 1 mM: 46% inactivation | Klebsiella pneumoniae | |
Zn2+ | extracellular enzyme, strong inhibition | Klebsiella pneumoniae | |
ZnSO4 | extracellular enzyme, strong inhibition | Klebsiella pneumoniae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell bound | - |
Klebsiella pneumoniae | - |
- |
extracellular | ultraviolet induced mutant | Klebsiella pneumoniae | - |
- |
extracellular | extracellular enzyme has the same characteristics as the cell-bound enzyme: enzyme is secreted extracellulary due to mutation of parent strain of Klebsiella pneumoniae | Klebsiella pneumoniae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | native enzyme, activation | Klebsiella pneumoniae | |
Ca2+ | extracellular enzyme, slight activation | Klebsiella pneumoniae | |
CaCl2 | extracellular enzyme, slight activation | Klebsiella pneumoniae | |
Co2+ | extracellular enzyme, slight activation | Klebsiella pneumoniae | |
CoCl2 | extracellular enzyme, slight activation | Klebsiella pneumoniae | |
Mn2+ | extracellular enzyme, slight activation | Klebsiella pneumoniae | |
MnSO4 | extracellular enzyme, slight activation | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
cell-bound and extracellular enzyme, gel filtration | Klebsiella pneumoniae |
65000 | - |
extracellular enzyme, SDS-PAGE | Klebsiella pneumoniae |
67000 | - |
cell-bound enzyme, SDS-PAGE | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
Aerobacter aerogenes | - |
Klebsiella pneumoniae | - |
ultraviolet-induced mutant | - |
Klebsiella pneumoniae | - |
ultraviolet-induced mutant from Aerobacter aerogenes IFO-3321 | - |
Klebsiella pneumoniae | - |
IFO-3321 | - |
Klebsiella pneumoniae IFO-3321 | - |
IFO-3321 | - |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella pneumoniae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(Glcalpha(1-4))m + H2O = (Glcalpha(1-4))m-6 + maltohexaose | reaction mechanism is exo | Klebsiella pneumoniae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture filtrate | - |
Klebsiella pneumoniae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.2 | - |
cell-bound enzyme | Klebsiella pneumoniae |
18.4 | - |
extracellular enzyme | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Klebsiella pneumoniae | maltohexaose + ? | - |
? | |
amylopectin beta-amylase limit dextrin + H2O | - |
Klebsiella pneumoniae | oligosaccharide | branched oligosaccharides containing 5 to 10 glucose units, B5-B10 | ? | |
amylose + H2O | short-chain amylose, DP, average degree of polymerization, = 23 | Klebsiella pneumoniae | maltohexaose | - |
? | |
amylose + H2O | reduced short-chain amylose: about 95% of the rate of short-chain amylose | Klebsiella pneumoniae | maltohexaose | - |
? | |
amylose + H2O | potato amylose: about 57% of the rate of soluble starch | Klebsiella pneumoniae | maltohexaose | - |
? | |
amylose + H2O | short-chain amylose, DP, average degree of polymerization, = 23 | Klebsiella pneumoniae IFO-3321 | maltohexaose | - |
? | |
amylose + H2O | reduced short-chain amylose: about 95% of the rate of short-chain amylose | Klebsiella pneumoniae IFO-3321 | maltohexaose | - |
? | |
glycogen + H2O | - |
Klebsiella pneumoniae | maltohexaose | - |
? | |
glycogen beta-amylase limit dextrin + H2O | - |
Klebsiella pneumoniae | oligosaccharide | branched oligosaccharides containing 5 to 10 glucose units, B5-B10 | ? | |
maltohexaose + H2O | poor substrate | Klebsiella pneumoniae | maltose + maltotetraose | main product | ? | |
additional information | no reaction on alpha-, beta- or gamma-cyclodextrin, pullulan and maltose | Klebsiella pneumoniae | ? | - |
? | |
additional information | substrate and product specificity, overview | Klebsiella pneumoniae | ? | - |
? | |
additional information | exo action pattern | Klebsiella pneumoniae | ? | - |
? | |
additional information | no reaction on alpha-, beta- or gamma-cyclodextrin, pullulan and maltose | Klebsiella pneumoniae IFO-3321 | ? | - |
? | |
additional information | substrate and product specificity, overview | Klebsiella pneumoniae IFO-3321 | ? | - |
? | |
additional information | exo action pattern | Klebsiella pneumoniae IFO-3321 | ? | - |
? | |
starch + H2O | soluble starch | Klebsiella pneumoniae | maltohexaose + ? | main product | ? | |
starch + H2O | waxy-maize starch | Klebsiella pneumoniae | maltohexaose + ? | main product | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | extracellular enzyme, 1 * 65000, SDS-PAGE | Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
52 | - |
extracellular enzyme | Klebsiella pneumoniae |
60 | - |
cell-bound enzyme | Klebsiella pneumoniae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme more thermostable in the presence of Ca2+ | Klebsiella pneumoniae |
50 | - |
cell-bound and extracellular enzyme, stable below 50°C, thermostability improved by addition of Ca2+ | Klebsiella pneumoniae |
55 | - |
extracellular enzyme, stable up to 55°C in the presence of 2 mM CaCl2 | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
cell-bound enzyme | Klebsiella pneumoniae |
7 | - |
extracellular enzyme | Klebsiella pneumoniae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | extracellular enzyme, more than 20% activity at pH 5.0, more than 50% activity at pH 10.0 | Klebsiella pneumoniae |
6 | 8 | extracellular enzyme, more than 80% of maximum activity between pH 6.0 and 8.0 | Klebsiella pneumoniae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH stability: extracellular enzyme is a little more stable compared with the cell-bound enzyme | Klebsiella pneumoniae |
additional information | - |
extracellular enzyme, relatively wide pH stability | Klebsiella pneumoniae |
3 | - |
extracellular enzyme, most of activity lost below pH 3.0 | Klebsiella pneumoniae |
5 | 10 | extracellular enzyme, more than 80% of activity retains | Klebsiella pneumoniae |
6 | 9 | cell-bound enzyme: 20% loss of activity, 60 min, 40°C | Klebsiella pneumoniae |
11 | - |
extracellular enzyme, most of activity lost above pH 11.0 | Klebsiella pneumoniae |