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Literature summary for 3.2.1.98 extracted from
- Purification and some properties of an extracellular exo-maltohexaohydrolase from an Aerobacter aerogenes mutant (1982), J. Jpn. Soc. Starch Sci., 29, 188-197.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| Ca2+ stabilizes, enzyme is more thermostable in the presence of Ca2+ | Klebsiella pneumoniae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | extracellular enzyme, strong inhibition | Klebsiella pneumoniae |  |
| AgNO3 | extracellular enzyme, strong inhibition | Klebsiella pneumoniae | |
| Cu2+ | extracellular enzyme, strong inhibition | Klebsiella pneumoniae | |
| CuSO4 | extracellular enzyme, strong inhibition | Klebsiella pneumoniae | |
| FeSO4 | extracellular enzyme | Klebsiella pneumoniae | |
| Hg2+ | extracellular enzyme, completely | Klebsiella pneumoniae | |
| HgCl2 | extracellular enzyme, completely | Klebsiella pneumoniae | |
| iodoacetamide | extracellular enzyme, 1 mM: 11% inactivation; slight inactivation | Klebsiella pneumoniae | |
| p-chloromercuribenzoate | extracellular enzyme, 1 mM: 46% inactivation | Klebsiella pneumoniae | |
| Zn2+ | extracellular enzyme, strong inhibition | Klebsiella pneumoniae | |
| ZnSO4 | extracellular enzyme, strong inhibition | Klebsiella pneumoniae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell bound | - |
Klebsiella pneumoniae | - |
- |
| extracellular | ultraviolet induced mutant | Klebsiella pneumoniae | - |
- |
| extracellular | extracellular enzyme has the same characteristics as the cell-bound enzyme: enzyme is secreted extracellulary due to mutation of parent strain of Klebsiella pneumoniae | Klebsiella pneumoniae | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | native enzyme, activation | Klebsiella pneumoniae | |
| Ca2+ | extracellular enzyme, slight activation | Klebsiella pneumoniae | |
| CaCl2 | extracellular enzyme, slight activation | Klebsiella pneumoniae | |
| Co2+ | extracellular enzyme, slight activation | Klebsiella pneumoniae | |
| CoCl2 | extracellular enzyme, slight activation | Klebsiella pneumoniae | |
| Mn2+ | extracellular enzyme, slight activation | Klebsiella pneumoniae | |
| MnSO4 | extracellular enzyme, slight activation | Klebsiella pneumoniae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
cell-bound and extracellular enzyme, gel filtration | Klebsiella pneumoniae |
| 65000 | - |
extracellular enzyme, SDS-PAGE | Klebsiella pneumoniae |
| 67000 | - |
cell-bound enzyme, SDS-PAGE | Klebsiella pneumoniae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella pneumoniae | - |
Aerobacter aerogenes | - |
| Klebsiella pneumoniae | - |
ultraviolet-induced mutant | - |
| Klebsiella pneumoniae | - |
ultraviolet-induced mutant from Aerobacter aerogenes IFO-3321 | - |
| Klebsiella pneumoniae | - |
IFO-3321 | - |
| Klebsiella pneumoniae IFO-3321 | - |
IFO-3321 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Klebsiella pneumoniae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (Glcalpha(1-4))m + H2O = (Glcalpha(1-4))m-6 + maltohexaose | reaction mechanism is exo | Klebsiella pneumoniae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Klebsiella pneumoniae | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
cell-bound enzyme | Klebsiella pneumoniae |
| 18.4 | - |
extracellular enzyme | Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Klebsiella pneumoniae | maltohexaose + ? | - |
? | |
| amylopectin beta-amylase limit dextrin + H2O | - |
Klebsiella pneumoniae | oligosaccharide | branched oligosaccharides containing 5 to 10 glucose units, B5-B10 | ? | |
| amylose + H2O | short-chain amylose, DP, average degree of polymerization, = 23 | Klebsiella pneumoniae | maltohexaose | - |
? | |
| amylose + H2O | reduced short-chain amylose: about 95% of the rate of short-chain amylose | Klebsiella pneumoniae | maltohexaose | - |
? | |
| amylose + H2O | potato amylose: about 57% of the rate of soluble starch | Klebsiella pneumoniae | maltohexaose | - |
? | |
| amylose + H2O | short-chain amylose, DP, average degree of polymerization, = 23 | Klebsiella pneumoniae IFO-3321 | maltohexaose | - |
? | |
| amylose + H2O | reduced short-chain amylose: about 95% of the rate of short-chain amylose | Klebsiella pneumoniae IFO-3321 | maltohexaose | - |
? | |
| glycogen + H2O | - |
Klebsiella pneumoniae | maltohexaose | - |
? | |
| glycogen beta-amylase limit dextrin + H2O | - |
Klebsiella pneumoniae | oligosaccharide | branched oligosaccharides containing 5 to 10 glucose units, B5-B10 | ? | |
| maltohexaose + H2O | poor substrate | Klebsiella pneumoniae | maltose + maltotetraose | main product | ? | |
| additional information | no reaction on alpha-, beta- or gamma-cyclodextrin, pullulan and maltose | Klebsiella pneumoniae | ? | - |
? | |
| additional information | substrate and product specificity, overview | Klebsiella pneumoniae | ? | - |
? | |
| additional information | exo action pattern | Klebsiella pneumoniae | ? | - |
? | |
| additional information | no reaction on alpha-, beta- or gamma-cyclodextrin, pullulan and maltose | Klebsiella pneumoniae IFO-3321 | ? | - |
? | |
| additional information | substrate and product specificity, overview | Klebsiella pneumoniae IFO-3321 | ? | - |
? | |
| additional information | exo action pattern | Klebsiella pneumoniae IFO-3321 | ? | - |
? | |
| starch + H2O | soluble starch | Klebsiella pneumoniae | maltohexaose + ? | main product | ? | |
| starch + H2O | waxy-maize starch | Klebsiella pneumoniae | maltohexaose + ? | main product | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | extracellular enzyme, 1 * 65000, SDS-PAGE | Klebsiella pneumoniae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 52 | - |
extracellular enzyme | Klebsiella pneumoniae |
| 60 | - |
cell-bound enzyme | Klebsiella pneumoniae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme more thermostable in the presence of Ca2+ | Klebsiella pneumoniae |
| 50 | - |
cell-bound and extracellular enzyme, stable below 50°C, thermostability improved by addition of Ca2+ | Klebsiella pneumoniae |
| 55 | - |
extracellular enzyme, stable up to 55°C in the presence of 2 mM CaCl2 | Klebsiella pneumoniae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
cell-bound enzyme | Klebsiella pneumoniae |
| 7 | - |
extracellular enzyme | Klebsiella pneumoniae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | extracellular enzyme, more than 20% activity at pH 5.0, more than 50% activity at pH 10.0 | Klebsiella pneumoniae |
| 6 | 8 | extracellular enzyme, more than 80% of maximum activity between pH 6.0 and 8.0 | Klebsiella pneumoniae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH stability: extracellular enzyme is a little more stable compared with the cell-bound enzyme | Klebsiella pneumoniae |
| additional information | - |
extracellular enzyme, relatively wide pH stability | Klebsiella pneumoniae |
| 3 | - |
extracellular enzyme, most of activity lost below pH 3.0 | Klebsiella pneumoniae |
| 5 | 10 | extracellular enzyme, more than 80% of activity retains | Klebsiella pneumoniae |
| 6 | 9 | cell-bound enzyme: 20% loss of activity, 60 min, 40°C | Klebsiella pneumoniae |
| 11 | - |
extracellular enzyme, most of activity lost above pH 11.0 | Klebsiella pneumoniae |
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Release 2023.1 (January 2023)
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