Literature summary for 3.2.1.98 extracted from

Nakakuki, T.; Hayashi, T.; Monma, M.; Kawashima, K.; Kainuma, K.
Immobilization of the exo-maltohexaohydrolase by the irradiation method (1983), Biotechnol. Bioeng., 25, 1095-1107.

Search for more literature for 3.2.1.98

Application

Application	Comment	Organism
synthesis	continuous production of maltohexaose on large scale using the immobilized exomaltohexaohydrolase	Klebsiella pneumoniae

General Stability

General Stability	Organism
immobilized enzyme is very stable	Klebsiella pneumoniae
immobilized enzyme stable to repeated use of 20 cycles	Klebsiella pneumoniae

Inhibitors

Inhibitors	Comment	Organism	Structure
AgNO3	native and immobilized enzyme, immobilized enzyme less than native enzyme	Klebsiella pneumoniae
Al2(SO4)3	immobilized enzyme, slight inactivation	Klebsiella pneumoniae
Ba2+	immobilized enzyme	Klebsiella pneumoniae
Cu2+	immobilized enzyme less than native enzyme	Klebsiella pneumoniae
FeCl3	immobilized enzyme	Klebsiella pneumoniae
FeSO4	native and immobilized enzyme	Klebsiella pneumoniae
Hg2+	immobilized enzyme, 61% inactivation; native enzyme, completely	Klebsiella pneumoniae
HgCl2	immobilized enzyme, 61% inactivation; native enzyme, completely	Klebsiella pneumoniae
iodoacetamide	native enzyme: 11% inhibition, immobilized enzyme: 4% inhibition	Klebsiella pneumoniae
Mg2+	immobilized enzyme, slight inactivation	Klebsiella pneumoniae
Mn2+	native enzyme, slight inactivation	Klebsiella pneumoniae
additional information	native enzyme compared with immobilized enzyme	Klebsiella pneumoniae
p-chloromercuribenzoate	native enzyme: 46% inhibition, immobilized enzyme: 15% inhibition	Klebsiella pneumoniae
SrCl2	immobilized enzyme	Klebsiella pneumoniae
Zn2+	native enzyme	Klebsiella pneumoniae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	native enzyme compared with immobilized enzyme, Km-value of native and immobilized enzyme for short-chain amylose is the same, for soluble starch Km-value of immobilized enzyme is larger than that of native enzyme	Klebsiella pneumoniae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	ultraviolet induced mutant	Klebsiella pneumoniae	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	native enzyme, activation	Klebsiella pneumoniae
Ca2+	immobilized enzyme, no activation	Klebsiella pneumoniae
CoCl2	native and immobilized enzyme, very slight activation	Klebsiella pneumoniae
Mn2+	immobilized enzyme, activation	Klebsiella pneumoniae
additional information	native enzyme compared with immobilized enzyme	Klebsiella pneumoniae
NiCl2	immobilized enzyme, slight activation	Klebsiella pneumoniae
Sr2+	native enzyme, very slight activation	Klebsiella pneumoniae
Zn2+	immobilized enzyme, very slight activation	Klebsiella pneumoniae

Organism

Organism	UniProt	Comment	Textmining
Klebsiella pneumoniae	-	Aerobacter aerogenes	-
Klebsiella pneumoniae	-	native and immobilized, by irradiation method, enzyme	-
Klebsiella pneumoniae	-	ultraviolet-induced mutant	-

Purification (Commentary)

Purification (Comment)	Organism
partial	Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
15.6	-	-	Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
amylose + H2O	short-chain amylose, DP, average degree of polymerization, = 23	Klebsiella pneumoniae	maltohexaose	main product, about 38% maltohexaose	?
maltohexaose + H2O	-	Klebsiella pneumoniae	maltose + maltotetraose	-	?
maltohexaose + H2O	-	Klebsiella pneumoniae	D-glucose + maltopentaose	-	?
maltohexaose + H2O	-	Klebsiella pneumoniae	maltotriose + ?	-	?
additional information	action pattern on substrates of native and immobilized enzyme is almost the same	Klebsiella pneumoniae	?	-	?
starch + H2O	-	Klebsiella pneumoniae	maltohexaose + ?	main product	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
additional information	-	native enzyme compared with immobilized enzyme	Klebsiella pneumoniae
52	-	immobilized enzyme	Klebsiella pneumoniae

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
additional information	-	native enzyme compared with immobilized enzyme	Klebsiella pneumoniae

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	thermostability is improved by immobilization	Klebsiella pneumoniae
50	-	immobilized enzyme, completely stable up to	Klebsiella pneumoniae
75	-	immobilized enzyme, 15 min at 75°C: 40% of activity retained	Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
additional information	-	native enzyme compared with immobilized enzyme, immobilized enzyme: optimum pH shifted slightly to acidic side	Klebsiella pneumoniae

pH Range

pH Minimum	pH Maximum	Comment	Organism
additional information	-	native enzyme compared with immobilized enzyme	Klebsiella pneumoniae
5	8	immobilized enzyme, more than 80% of maximum activity between 5.0 and 8.0	Klebsiella pneumoniae

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
additional information	-	pH stability is improved by immobilization	Klebsiella pneumoniae
3	-	immobilized enzyme, 93% of activity retains	Klebsiella pneumoniae
4	11	immobilized enzyme, stable between	Klebsiella pneumoniae
11.5	-	immobilized enzyme, 85% of activity retained	Klebsiella pneumoniae

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Release 2023.1 (January 2023)