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Literature summary for 3.2.1.97 extracted from

  • Kiyohara, M.; Nakatomi, T.; Kurihara, S.; Fushinobu, S.; Suzuki, H.; Tanaka, T.; Shoda, S.; Kitaoka, M.; Katayama, T.; Yamamoto, K.; Ashida, H.
    alpha-N-acetylgalactosaminidase from infant-associated bifidobacteria belonging to novel glycoside hydrolase family 129 is implicated in alternative mucin degradation pathway (2012), J. Biol. Chem., 287, 693-700.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)DELTAlacZ cells Bifidobacterium bifidum

Inhibitors

Inhibitors Comment Organism Structure
additional information not affected by Mg2+, Ca2+, Co2+,Ni2+, and EDTA Bifidobacterium bifidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2
-
 Tn antigen at pH 5.0 and 37°C Bifidobacterium bifidum
17.1
-
 4,6-dimethoxy-1,3,5-triazin-2-yl alpha-N-acetylgalactosaminide at pH 5.0 and 37°C Bifidobacterium bifidum

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 7% residual activity at 5 mM Bifidobacterium bifidum
Mn2+ 75% residual activity at 5 mM Bifidobacterium bifidum
Zn2+ 30% residual activity at 5 mM Bifidobacterium bifidum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bifidobacterium bifidum the enzyme acts very weakly on GalNAcalpha1-UDP and GalNAcalpha(1–>3)Galbeta(1–>4)Glc and not at all on Neu5Acalpha(2–>6)GalNAcalpha1-L-Ser (sialyl Tn antigen)and GalNAcalpha(1–>3)(Fucalpha(1–>2))Gal (blood group A trisaccharide). In addition, the enzyme very slowly hydrolyzes core-3 (4-nitrophenyl-GlcNAcbeta(1–>3)GalNAcalpha1) and core-8 (4-nitrophenyl-Galalpha(1–>3)GalNAcalpha1)-type structures but not other core structures ?
-
 ?
Tn antigen + H2O Bifidobacterium bifidum i.e. N-acetyl-beta-D-galactosaminyl-L-Ser/Thr ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bifidobacterium bifidum G5ELM1
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 200 gel filtration Bifidobacterium bifidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4,6-dimethoxy-1,3,5-triazin-2-yl alpha-N-acetylgalactosaminide + H2O
-
 Bifidobacterium bifidum ?
-
 ?
4-nitrophenyl-N-acetyl-beta-D-galactosaminide + H2O
-
 Bifidobacterium bifidum 4-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?
4-nitrophenyl-N-acetyl-beta-D-galactosaminide + L-Ser transglycosylation Bifidobacterium bifidum ?
-
 ?
additional information the enzyme acts very weakly on GalNAcalpha1-UDP and GalNAcalpha(1–>3)Galbeta(1–>4)Glc and not at all on Neu5Acalpha(2–>6)GalNAcalpha1-L-Ser (sialyl Tn antigen)and GalNAcalpha(1–>3)(Fucalpha(1–>2))Gal (blood group A trisaccharide). In addition, the enzyme very slowly hydrolyzes core-3 (4-nitrophenyl-GlcNAcbeta(1–>3)GalNAcalpha1) and core-8 (4-nitrophenyl-Galalpha(1–>3)GalNAcalpha1)-type structures but not other core structures Bifidobacterium bifidum ?
-
 ?
Tn antigen + H2O i.e. N-acetyl-beta-D-galactosaminyl-L-Ser/Thr Bifidobacterium bifidum ?
-
 ?

Synonyms

Synonyms Comment Organism
endo-alpha-N-acetylgalactosaminidase
-
 Bifidobacterium bifidum
NagBb
-
 Bifidobacterium bifidum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Bifidobacterium bifidum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 the enzyme is stable up to 45°C Bifidobacterium bifidum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16.7
-
 4,6-dimethoxy-1,3,5-triazin-2-yl alpha-N-acetylgalactosaminide at pH 5.0 and 37°C Bifidobacterium bifidum
47.6
-
 Tn antigen at pH 5.0 and 37°C Bifidobacterium bifidum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Bifidobacterium bifidum

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 11 the enzyme is stable over the pH range of 3.0-11.0 Bifidobacterium bifidum