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Literature summary for 3.2.1.97 extracted from
- Characterization of two different endo-alpha-N-acetylgalactosaminidases from probiotic and pathogenic enterobacteria, Bifidobacterium longum and Clostridium perfringens (2008), Glycobiology, 18, 727-734.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| EngCP expressed in Escherichia coli | Clostridium perfringens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | is severely inhibited by 1 mM | Clostridium perfringens |  |
| additional information | EngBF is not significantly inhibited by divalent cations | Bifidobacterium longum | |
| additional information | 1 mM of Ca2+, Zn2+, Co2+, Mn2+, Mg2+, and Fe2+, or the chelating reagent EDTA have no effect on activity | Clostridium perfringens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.051 | - |
Galbeta1-3GalNAcalpha1-pNP | - |
Clostridium perfringens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium longum | - |
- |
- |
| Clostridium perfringens | Q8XMJ5 | - |
- |
| Clostridium perfringens 13 | Q8XMJ5 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside + H2O | 1.8% relative activity | Clostridium perfringens | 4-nitrophenol + N-acetyl-alpha-D-galactosamine | - |
? | |
| 4-nitrophenyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside + H2O | 1.8% relative activity | Clostridium perfringens 13 | 4-nitrophenol + N-acetyl-alpha-D-galactosamine | - |
? | |
| asialofetuin + H2O | - |
Clostridium perfringens | ? | - |
? | |
| asialofetuin + H2O | - |
Clostridium perfringens 13 | ? | - |
? | |
| Galbeta1-3(GlcNAcbeta1-6)GalNAcalpha1-pNP + H2O | 2.3% relative activity | Clostridium perfringens | ? | - |
? | |
| Galbeta1-3(GlcNAcbeta1-6)GalNAcalpha1-pNP + H2O | 2.3% relative activity | Clostridium perfringens 13 | ? | - |
? | |
| Galbeta1-3GalNAcalpha1-pNP + H2O | 100% relative activity | Bifidobacterium longum | ? | - |
? | |
| Galbeta1-3GalNAcalpha1-pNP + H2O | 100% relative activity | Clostridium perfringens | ? | - |
? | |
| Galbeta1-3GalNAcalpha1-pNP + H2O | 100% relative activity | Clostridium perfringens 13 | ? | - |
? | |
| GalNAcbeta1-3GalNAcalpha1-pNP + H2O | 17.3% relative activity | Bifidobacterium longum | ? | - |
? | |
| GalNAcbeta1-3GalNAcalpha1-pNP + H2O | 21% relative activity | Clostridium perfringens | ? | - |
? | |
| gastric mucin + H2O | EngBF does not release oligosaccharides from intact gastric mucin, but releases core 1 disaccharide from the gastric mucin pretreated with commercial sialidase and with the recombinant exo-alpha-N-acetylglucosamindase | Bifidobacterium longum | disaccharide + ? | - |
? | |
| gastric mucin + H2O | EngCP does not release oligosaccharides from intact gastric mucin, but releases core 1 disaccharide from the gastric mucin pretreated with commercial sialidase and with the recombinant exo-alpha-N-acetylglucosamindase cloned from the same strain of Clostridium perfringens. It does not release core 2 trisaccharide from the mucin | Clostridium perfringens | disaccharide + ? | - |
? | |
| Glcbeta1-3GalNAcalpha1-pNP + H2O | 156.7% relative activity | Clostridium perfringens | ? | - |
? | |
| Glcbeta1-3GalNAcalpha1-pNP + H2O | 35.3% relative activity | Bifidobacterium longum | ? | - |
? | |
| GlcNAcbeta1-3GalNAcalpha1-pNP + H2O | 0.3% relative activity | Bifidobacterium longum | ? | - |
? | |
| GlcNAcbeta1-3GalNAcalpha1-pNP + H2O | 3.5% relative activity | Clostridium perfringens | ? | - |
? | |
| additional information | EngCP from Clostridium perfringens possesses broader substrate specificity than EngBF | Bifidobacterium longum | ? | - |
? | |
| additional information | EngCP possesses broader substrate specificity than EngBF of Bifidobacterium longum, which may contribute to the pathogenicity of Clostridium perfringens. EngCP does not act on sialyl core 1 O-glycans, e.g., fetuin | Clostridium perfringens | ? | - |
? | |
| additional information | EngCP possesses broader substrate specificity than EngBF of Bifidobacterium longum, which may contribute to the pathogenicity of Clostridium perfringens. EngCP does not act on sialyl core 1 O-glycans, e.g., fetuin | Clostridium perfringens 13 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-alpha-GalNAc-ase | - |
Bifidobacterium longum |
| endo-alpha-GalNAc-ase | - |
Clostridium perfringens |
| endo-alpha-N-acetylgalactosaminidase | - |
Bifidobacterium longum |
| endo-alpha-N-acetylgalactosaminidase | - |
Clostridium perfringens |
| EngBF | - |
Bifidobacterium longum |
| EngCP | - |
Clostridium perfringens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Clostridium perfringens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 110 | - |
Galbeta1-3GalNAcalpha1-pNP | - |
Clostridium perfringens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
optimal pH between pH 5-6 for all hydrolyzable substrates of EngBF | Bifidobacterium longum |
| 6 | - |
optimum pH of the enzyme for Galbeta1-3GalNAcalpha1-pNP hydrolysis is at pH 6.0 and 9.0, whereas optimum pH for other substrates hydrolyzed is around pH 6.0 | Clostridium perfringens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | retains more than 80% activity | Clostridium perfringens |
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