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Literature summary for 3.2.1.97 extracted from

  • Katayama, T.; Fujita, K.; Yamamoto, K.
    Novel bifidobacterial glycosidases acting on sugar chains of mucin glycoproteins (2005), J. Biosci. Bioeng., 99, 457-465.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bifidobacterium longum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.165
-
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol pH 5.0, 37°C Bifidobacterium longum

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
Bifidobacterium longum 9986
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
210000
-
predicted from amino acid sequence Bifidobacterium longum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O Bifidobacterium bifidum
-
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O Bifidobacterium breve
-
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O Bifidobacterium longum
-
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O Bifidobacterium breve JCM 1192
-
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O Bifidobacterium bifidum ATCC 29521
-
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O Bifidobacterium longum JCM 7054
-
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O Bifidobacterium longum JCM 1217
-
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O Bifidobacterium bifidum JCM 7004
-
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O Bifidobacterium bifidum
-
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O Bifidobacterium breve
-
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O Bifidobacterium longum
-
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O Bifidobacterium breve JCM 1192
-
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O Bifidobacterium bifidum ATCC 29521
-
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O Bifidobacterium longum JCM 7054
-
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O Bifidobacterium longum JCM 1217
-
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O Bifidobacterium bifidum JCM 7004
-
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
?

Organism

Organism UniProt Comment Textmining
Bifidobacterium bifidum
-
ATCC29521
-
Bifidobacterium bifidum
-
JCM 1254
-
Bifidobacterium bifidum
-
JCM 7004
-
Bifidobacterium bifidum ATCC 29521
-
ATCC29521
-
Bifidobacterium bifidum JCM 7004
-
JCM 7004
-
Bifidobacterium breve
-
JCM 1192
-
Bifidobacterium breve JCM 1192
-
JCM 1192
-
Bifidobacterium longum
-
JCM 7054
-
Bifidobacterium longum Q3T552 JCM 1217
-
Bifidobacterium longum JCM 1217 Q3T552 JCM 1217
-
Bifidobacterium longum JCM 7054
-
JCM 7054
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Bifidobacterium longum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
-
Bifidobacterium bifidum 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
-
Bifidobacterium breve 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
-
Bifidobacterium longum 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
-
Bifidobacterium breve JCM 1192 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
-
Bifidobacterium bifidum ATCC 29521 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
-
Bifidobacterium longum JCM 7054 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
-
Bifidobacterium longum JCM 1217 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
-
Bifidobacterium bifidum JCM 7004 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
-
Bifidobacterium bifidum 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
-
Bifidobacterium breve 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
-
Bifidobacterium longum 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
-
Bifidobacterium breve JCM 1192 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
-
Bifidobacterium bifidum ATCC 29521 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
-
Bifidobacterium longum JCM 7054 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
-
Bifidobacterium longum JCM 1217 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
-
Bifidobacterium bifidum JCM 7004 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
?
Gal-beta-1,3-GalNAc-alpha-1p-nitrophenol + H2O pH 5.0, 37°C Bifidobacterium longum p-nitrophenol + Gal-beta-1,3-GalNAc
-
?
Gal-beta-1,3-GalNAc-alpha-1p-nitrophenol + H2O pH 5.0, 37°C Bifidobacterium longum JCM 7054 p-nitrophenol + Gal-beta-1,3-GalNAc
-
?
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O pH 5.0, 37°C Bifidobacterium bifidum p-nitrophenol + Galbeta(1,3)GalNAc
-
?
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O pH 5.0, 37°C Bifidobacterium breve p-nitrophenol + Galbeta(1,3)GalNAc
-
?
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O pH 5.0, 37°C Bifidobacterium longum p-nitrophenol + Galbeta(1,3)GalNAc
-
?
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O pH 5.0, 37°C Bifidobacterium breve JCM 1192 p-nitrophenol + Galbeta(1,3)GalNAc
-
?
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O pH 5.0, 37°C Bifidobacterium bifidum ATCC 29521 p-nitrophenol + Galbeta(1,3)GalNAc
-
?
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O pH 5.0, 37°C Bifidobacterium longum JCM 1217 p-nitrophenol + Galbeta(1,3)GalNAc
-
?
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O pH 5.0, 37°C Bifidobacterium bifidum JCM 7004 p-nitrophenol + Galbeta(1,3)GalNAc
-
?
additional information Galbeta(1,3)GalNAc disaccharide is liberated from asialofetuin and p-nitrophenol substrate containing the core 1 structure (Galbeta(1,3)GalNAcalpha-1), but not from sialofetuin or any p-nitrophenol substrate other than Galbeta(1,3)GalNAcalpha-1-p-nitrophenol Bifidobacterium longum ?
-
?
additional information Galbeta(1,3)GalNAc disaccharide is liberated from asialofetuin and p-nitrophenol substrate containing the core 1 structure (Galbeta(1,3)GalNAcalpha-1), but not from sialofetuin or any p-nitrophenol substrate other than Galbeta(1,3)GalNAcalpha-1-p-nitrophenol Bifidobacterium longum JCM 1217 ?
-
?

Subunits

Subunits Comment Organism
? x * 210000, SDS-PAGE Bifidobacterium longum

Synonyms

Synonyms Comment Organism
endo-alpha-GalNAc-ase
-
Bifidobacterium bifidum
endo-alpha-GalNAc-ase
-
Bifidobacterium breve
endo-alpha-GalNAc-ase
-
Bifidobacterium longum
endo-alpha-N-acetylgalactosaminidase
-
Bifidobacterium bifidum
endo-alpha-N-acetylgalactosaminidase
-
Bifidobacterium breve
endo-alpha-N-acetylgalactosaminidase
-
Bifidobacterium longum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Bifidobacterium longum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
the enzyme is stable up to 37°C and retains 73% activity after incubation at 45°C for 30 min in 50 mM acetate buffer (pH 5.0) Bifidobacterium longum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
37
-
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol pH 5.0, 37°C Bifidobacterium longum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
at 37°C in 10 mM acetate buffer Bifidobacterium longum

pH Range

pH Minimum pH Maximum Comment Organism
4 6 68% activity at pH 4.0, 73% activity at pH 6.0 Bifidobacterium longum