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Literature summary for 3.2.1.97 extracted from

  • Ashida, H.; Yamamoto, K.; Murata, T.; Usui, T.; Kumagai, H.
    Characterization of endo-alpha-N-acetylgalactosaminidase from Bacillus sp. and syntheses of neo-oligosaccharides using its transglycosylation activity (2000), Arch. Biochem. Biophys., 373, 394-400.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
Fe2+ 1 mM Bacillus sp. (in: Bacteria)
Hg2+
-
 Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 gel filtration Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30.1
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
asialo kappa-casein + H2O
-
 Bacillus sp. (in: Bacteria) Galbeta(1-3)GalNAc + ?
-
 ?
asialofetuin + H2O
-
 Bacillus sp. (in: Bacteria) additional information
-
 ?
asialoglycophorin + H2O asialoglycophorin A Bacillus sp. (in: Bacteria) Galbeta(1-3)GalNAc + ?
-
 ?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + galactose transglycosylation Bacillus sp. (in: Bacteria) ?
-
 ?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + glucose transglycosylation Bacillus sp. (in: Bacteria) ?
-
 ?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + H2O
-
 Bacillus sp. (in: Bacteria) Galbeta(1-3)GalNAc + p-nitrophenol
-
 ?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + maltose transglycosylation Bacillus sp. (in: Bacteria) ?
-
 ?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + mannitol transglycosylation Bacillus sp. (in: Bacteria) ?
-
 ?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + mannose transglycosylation Bacillus sp. (in: Bacteria) ?
-
 ?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + sorbitol transglycosylation Bacillus sp. (in: Bacteria) ?
-
 ?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + sucrose transglycosylation Bacillus sp. (in: Bacteria) ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 110000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 10 min, stable Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 37°C, 30 min, stable Bacillus sp. (in: Bacteria)