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Literature summary for 3.2.1.97 extracted from

  • Fan, J.Q.; Kadowaki, S.; Yamamoto, K.; Kumagai, H.; Tochikura, T.
    Purification and characterization of endo-alpha-N-acetylgalactosaminidase from Alcaligenes sp. (1988), Agric. Biol. Chem., 52, 1715-1723.
No PubMed abstract available
Search for more literature for 3.2.1.97

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 2.5 mM, 26% inhibition Alcaligenes sp.
Cys 2.5 mM, 32% inhibition Alcaligenes sp.
Hg2+ 2.5 mM, complete inhibition Alcaligenes sp.
Mn2+ 2.5 mM, 35% inhibition Alcaligenes sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2
-
 asialo kappa-casein
-
 Alcaligenes sp.
3.7
-
 asialofetuin
-
 Alcaligenes sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 gel filtration Alcaligenes sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Alcaligenes sp. highly induced by porcine gastric mucin ?
-
 ?
additional information Alcaligenes sp. F-1906 highly induced by porcine gastric mucin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Alcaligenes sp.
-
-
-
Alcaligenes sp. F-1906
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Alcaligenes sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Alcaligenes sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.18
-
-
 Alcaligenes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
asialo kappa-casein + H2O
-
 Alcaligenes sp. Galbeta(1-3)GalNAc + ?
-
 ?
asialo kappa-casein + H2O
-
 Alcaligenes sp. F-1906 Galbeta(1-3)GalNAc + ?
-
 ?
asialofetuin + H2O
-
 Alcaligenes sp. additional information Galbeta(1-3)GalNAc + ? ?
asialofetuin + H2O
-
 Alcaligenes sp. F-1906 additional information Galbeta(1-3)GalNAc + ? ?
asialoglycophorin + H2O
-
 Alcaligenes sp. Galbeta(1-3)GalNAc + ?
-
 ?
asialoglycophorin + H2O
-
 Alcaligenes sp. F-1906 Galbeta(1-3)GalNAc + ?
-
 ?
glycoprotein + H2O release of Galbeta(1-3)GalNAc from glycoproteins possesing Ser or Thr O-glycosidic linkages Alcaligenes sp. Galbeta(1-3)GalNAc + ?
-
 ?
glycoprotein + H2O release of Galbeta(1-3)GalNAc from glycoproteins possesing Ser or Thr O-glycosidic linkages Alcaligenes sp. F-1906 Galbeta(1-3)GalNAc + ?
-
 ?
additional information highly induced by porcine gastric mucin Alcaligenes sp. ?
-
 ?
additional information highly induced by porcine gastric mucin Alcaligenes sp. F-1906 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 160000, SDS-PAGE Alcaligenes sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
 Alcaligenes sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 10 min, stable below Alcaligenes sp.
55
-
 10 min, complete inactivation Alcaligenes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5
-
 Alcaligenes sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 9 about 50% of maximal activity at pH 4.0 and at pH 9.0 Alcaligenes sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 6.5 30°C, 1 h, stable Alcaligenes sp.