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Literature summary for 3.2.1.97 extracted from
- Purification and properties of an endo-alpha-N-acetyl-D-galactosaminidase from Diplococcus pneumoniae (1977), J. Biol. Chem., 252, 8609-8614.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | the enzyme is ideally suited to explore the distribution and function of mucin-type glycoproteins on normal and cancer cell surfaces | Streptococcus pneumoniae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cys | 2 mM, 12% inhibition | Streptococcus pneumoniae |  |
| EDTA | 2 mM, 40% inhibition | Streptococcus pneumoniae | |
| Hg2+ | 2 mM, complete inhibition | Streptococcus pneumoniae | |
| Mg2+ | 2 mM, 28% inhibition | Streptococcus pneumoniae | |
| Mn2+ | 2 mM, 29% inhibition | Streptococcus pneumoniae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
asialofetuin | - |
Streptococcus pneumoniae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 160000 | - |
gel filtration | Streptococcus pneumoniae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus pneumoniae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptococcus pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| asialofetuin + H2O | - |
Streptococcus pneumoniae | additional information | - |
? | |
| glycopeptide + H2O | specific requirement for an unsubstituted galactose at the nonreducing terminus and an alpha-linkage between N-acetylgalactosamine and the aglycone | Streptococcus pneumoniae | Galbeta(1-3)GalNAc + ? | - |
? | |
| glycopeptide + H2O | release of Galbeta(1-3)GalNAc from glycopeptides possesing Ser or Thr O-glycosidic linkages | Streptococcus pneumoniae | Galbeta(1-3)GalNAc + ? | - |
? | |
| glycoprotein + H2O | specific requirement for an unsubstituted galactose in the nonreducing terminus and an alpha-linkage between N-acetylgalactosamine and the aglycone | Streptococcus pneumoniae | Galbeta(1-3)GalNAc + ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Streptococcus pneumoniae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.8 | pH 6.0: about 40% of maximal activity, pH 8.8: about 60% of maximal activity | Streptococcus pneumoniae |
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Release 2023.1 (January 2023)
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